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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Green tea extract improves high fat diet-induced hypothalamic inflammation, without affecting the serotoninergic system

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Author(s):
Okuda, Marcos H. [1] ; Zemdegs, Juliane C. S. [1] ; de Santana, Aline A. [1] ; Santamarina, Aline B. [1] ; Moreno, Mayara F. [1] ; Hachul, Ana C. L. [1] ; dos Santos, Bruno [1] ; Oller do Nascimento, Claudia M. [1] ; Ribeiro, Eliane B. [1] ; Oyama, Lila M. [1]
Total Authors: 10
Affiliation:
[1] Univ Fed Sao Paulo, Disciplina Fisiol Nutr, Dept Fisiol, BR-04023062 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 25, n. 10, p. 1084-1089, OCT 2014.
Web of Science Citations: 18
Abstract

To investigate possible mechanisms of green tea's anti-obesity and anti-diabetic effects in the hypothalamus, the central regulator of metabolism, of mice fed with high-fat diet (HFD), we analyzed proteins of the toll-like receptor 4 (TLR4) pathway and serotoninergic proteins involved in energy homeostasis. Thirty-day-old male Swiss mice were fed with HFD rich in saturated fat and green tea extract (GTE) for 8 weeks. After that, body weight and mass of fat depots were evaluated. Oral glucose tolerance test was performed 3 days prior to euthanasia; serum glucose, insulin and adiponectin were measured in fasted mice. Hypothalamic TLR4 pathway proteins, serotonin receptors 1B and 2C and serotonin transporter were analyzed by Western blotting or enzyme-linked immunosorbent assay. A second set of animals was used to measure food intake in response to fluoxetine, a selective serotonin reuptake inhibitor. Mice fed with HFD had increased body weight and mass of fat depots, impaired oral glucose tolerance, elevated glucose and insulin and decreased adiponectin serum levels. TLR4, I kappa B-alpha, nuclear factor kappa B p50 and interleukin 6 were increased by HFD. Concomitant GTE treatment ameliorated these parameters. The serotoninergic system remained functional after HFD treatment despite a few alterations in protein content of serotonin receptors I B and 2C and serotonin transporter. In summary, the GTE attenuated the deleterious effects of the HFD investigated in this study, partially due to reduced hypothalamic inflammation. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 09/14373-8 - Effect of epigallocatechin-gallate on adipokine secretion and TLR4 and NF-kB signalling in adipocytes: in vivo and in vitro study
Grantee:Lila Missae Oyama
Support type: Regular Research Grants
FAPESP's process: 12/03713-5 - Effect of green tea extract rich in epigalocatechin-galate intake on citokines and metabolic regulators in adipose tissue and esqueletic muscle in obese mice induced by hyperlilipidic diet
Grantee:Lila Missae Oyama
Support type: Regular Research Grants
FAPESP's process: 11/16199-5 - Effect of prolonged treatment with epigallocatechin-3-gallate on the hippocampal and hypothalamic content of proteins related to inflammation and serotonin pathways.
Grantee:Marcos Hiromu Okuda
Support type: Scholarships in Brazil - Master