Detection and Quantification of Periodontal Pathogens in Smokers and Never-Smokers With Chronic Periodontitis by Real-Time Polymerase Chain Reaction

Autor(es)

Guglielmetti, Mariana R. ^[1] ; Rosa, Ecinele F. ^[1] ; Lourencao, Daniele S. ^[1] ; Inoue, Gislene ^[1] ; Gomes, Elaine F. ^[1] ; De Micheli, Giorgio ^[1] ; Mendes, Fausto Medeiros ^[1] ; Hirata, Rosario D. C. ^[2] ; Hirata, Mario H. ^[2] ; Pannuti, Claudio M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Periodontol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin & Toxicol Anal, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Periodontology; v. 85, n. 10, p. 1450-1457, OCT 2014.
• Citações Web of Science: 18

Resumo

Background: The purpose of the present investigation is to compare the presence and number of periodontal pathogens in the subgingival microbiota of smokers versus never-smokers with chronic periodontitis and matched probing depths (PDs) using real-time polymerase chain reaction (RT-PCR). Methods: Forty current smokers and 40 never-smokers, matched for age, sex, and mean PD of sampling site, were included in this investigation. A full-mouth periodontal examination was performed, and a pooled subgingival plaque sample was collected from the deepest site in each quadrant of each participant. To confirm smoking status, expired carbon monoxide (CO) concentrations were measured with a CO monitor. The presence and quantification of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were determined using RT-PCR. Results: Smokers had greater overall mean PD (P = 0.001) and attachment loss (P = 0.006) and fewer bleeding on probing sites (P = 0.001). An association was observed between smoking status and the presence of A. actinomycetemcomitans (P <0.001). The counts of A. actinomycetemcomitans (P <0.001), P. gingivalis (P = 0.042), and T. forsythia (P <0.001) were significantly higher in smokers. Conclusions: Smokers showed significantly greater amounts of P. gingivalis, A. actinomycetemcomitans, and T. forsythia than never-smokers. There was a significant association between smoking and the presence of A. actinomycetemcomitans. (AU)

• Processo FAPESP: 11/02975-3 - Efeito do abandono do tabagismo na microbiota periodontal de fumantes
• Beneficiário: Cláudio Mendes Pannuti
• Modalidade de apoio: Auxílio à Pesquisa - Regular