Maternal Western Style Diet Increases Susceptibility to Chemically-Induced Mammary Carcinogenesis in Female Rats Offspring

Autor(es)

Lopes, Gisele A. D. ^[1] ; Fan, William Y. C. ^[1] ; Ciol, Heloisa ^[2] ; Bidinotto, Lucas T. ^{[3, 4]} ; Rodrigues, Maria A. M. ^[1] ; Barbisan, Luis F. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Botucatu Med Sch, Dept Pathol, Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Botucatu Biosci Inst, Dept Morphol, Sao Paulo - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, Sao Paulo - Brazil

^[4] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL; v. 66, n. 8, p. 1293-1303, NOV 17 2014.
• Citações Web of Science: 2

Resumo

The present study investigated whether maternal exposure to western style diet (WD) increases susceptibility to mammary carcinogenesis induced by N-methyl-N-nitrosourea (MNU) in female offspring. Pregnant female Sprague-Dawley rats received WD diet or control diet from gestational day 12 until postnatal day (PND) 21. At PND 21, female offspring received a single dose of MNU (50mg/kg body weight) and were fed chow diet until PND 110. Mammary gland structures were assessed on whole-mount preparations in the offspring at PND 21, and tumor morphology was examined at PND 110. Immunohistochemical analysis for cell proliferation (PCNA), apoptosis (cleaved caspase-3) and estrogen receptor alpha (ER-alpha) was performed in mammary terminal end buds (TEBs) at PND 21, and PCNA, ER-alpha, and p63 analysis in mammary tumors at PND 110. Maternal WD intake induced a significant increase in the number of TEBs (P = 0.024) and in PCNA labeling index (P < 0.020) in the mammary glands at PND 21. Tumor multiplicity, tumor weight, and PCNA labeling indexes were significantly higher in the WD offspring than that of the control offspring (P < 0.05). These findings indicate that maternal western style diet potentially enhanced the development of mammary tumors induced by MNU in female offspring, possibly by affecting the mammary gland differentiation. (AU)

• Processo FAPESP: 07/57408-0 - Exposicao a dieta ocidental durante a gestacao, lactacao, fase juvenil e susceptibilidade a carcinogenese quimica do colon em ratos adultos da linhagem sprague dawley.
• Beneficiário: Gisele Aparecida Dionísio Lopes
• Modalidade de apoio: Bolsas no Brasil - Mestrado