Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Effect of corticosteroids and lung ventilation in the VEGF and NO pathways in congenital diaphragmatic hernia in rats

Texto completo
Autor(es):
Lanhellas Goncalves, Frances Lilian [1] ; Figueira, Rebeca Lopes [1] ; Simoes, Ana Leda Bertoncini [1] ; Gallindo, Rodrigo Melo [1] ; Coleman, Allan [2] ; Peiro, Jose Luis [2] ; Sbragia, Lourenco [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Lab Expt Fetal Surg Michael R Harrison, Div Pediat Surg, Dept Surg & Anat, BR-14048900 Ribeirao Preto, SP - Brazil
[2] Univ Cincinnati, Cincinnati Childrens Hosp Med Ctr, Cincinnati, OH - USA
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: PEDIATRIC SURGERY INTERNATIONAL; v. 30, n. 12, p. 1207-1215, DEC 2014.
Citações Web of Science: 5
Resumo

The use of dexamethasone (Dx) stimulates growth, fetal lung maturation and can improve pulmonary hypertension in congenital diaphragmatic hernia (CDH). Our aim was to evaluate the effect of Dx on the lung after fetal pulmonary ventilation in the CDH rat model. Some groups underwent prenatal treatment with dexamethasone (0.4 mg/kg) that was given at 18.5 gestational day (GD). Sprague-Dawley rat fetuses were divided into groups: control (C); ventilated control (CV); control exposed to dexamethasone (CDx); ventilated control exposed to dexamethasone (CVDx); congenital diaphragmatic hernia (CDH), ventilated CDH (CDHV), CDH exposed to dexamethasone (CDHDx) and ventilated CDH exposed to dexamethasone (CDHVDx). At 21.5 GD fetuses were delivered by C-section, weighed and ventilated for 30 min. We analyzed the lung morphometry by Masson's Trichrome stain, and VEGF, VEGFR1, VEGFR2 and NOS3 expression by immunohistochemistry. All fetuses with CDH, with or without prenatal dexamethasone showed lung and body weight lower than control fetuses (p < 0.05). All groups that received dexamethasone showed a decrease in the medial muscular layer of arterioles, the internal diameter of the air spaces (Lma) and length of parenchymal transection/airspace ratio (p < 0.05). In the immunohistochemistry, VEGF decreased more in CDHDV group (p < 0.05). VEGFR1 showed no difference, whereas VEGFR2 decreased significantly in the CDHDV group (p < 0.05). NOS3 increased in the group CDHDV (p < 0.05). The use of prenatal dexamethasone added to ventilation alters the VEGF and NO pathways. (AU)

Processo FAPESP: 11/12587-0 - Avaliação in vivo do efeito do corticóide e da ventilação na via do óxido nítrico sobre a expressão do VEGF e seus receptores pulmonares após a oclusão traqueal fetal na hérnia diafragmática congênita em ratos
Beneficiário:Frances Lilian Lanhellas Gonçalves
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/00794-1 - Avaliação da via do óxido nítrico in vivo após a oclusão traqueal fetal sobre a expressão de receptores de VEGF pulmonar na hérnia diafragmática congênita em ratos induzida pelo Nitrofen
Beneficiário:Lourenço Sbragia Neto
Linha de fomento: Auxílio à Pesquisa - Regular