Improving safety of salami by application of bacteriocins produced by an autochthonous Lactobacillus curvatus isolate

Autor(es)

Barbosa, Matheus de Souza ^[1] ; Todorov, Svetoslav Dimitrov ^[1] ; Ivanova, Iskra ^{[2, 3]} ; Chobert, Jean-Marc ^[2] ; Haertle, Thomas ^[2] ; Gombossy de Melo Franco, Bernadette Dora ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Sao Paulo - Brazil

^[2] Inst Natl Rech Agronom, UR Biopolymeres Interact Assemblages 1268, Equipe Fonct & Interact Prot, F-44316 Nantes 3 - France

^[3] Univ Sofia, Fac Biol Sci, Dept Microbiol, BU-1126 Sofia - Bulgaria

• Tipo de documento: Artigo Científico
• Fonte: FOOD MICROBIOLOGY; v. 46, p. 254-262, APR 2015.
• Citações Web of Science: 31

Resumo

The aims of this study were to isolate LAB with anti-Listeria activity from salami samples, characterize the bacteriocin/s produced by selected isolates, semi-purify them and evaluate their effectiveness for the control of Listeria monocytogenes during manufacturing of salami in a pilot scale. Two isolates (differentiated by RAPD-PCR) presented activity against 22 out of 23 L. monocytogenes strains for bacteriocin MBSa2, while the bacteriocin MBSa3 inhibited all 23 strains in addition to several other Gram-positive bacteria for both antimicrobials and were identified as Lactobacillus curvatus based on 16S rRNA sequencing. A three-step purification procedure indicated that both strains produced the same two active peptides (4457.9 Da and 4360.1 Da), homlogous to sakacins P and X, respectively. Addition of the semi-purified bacteriocins produced by Lb. curvatus MBSa2 to the batter for production of salami, experimentally contaminated with L. monocytogenes (10(4)-10(5) CFU/g), caused 2 log and 1.5 log reductions in the counts of the pathogen in the product after 10 and 20 days respectively, highlighting the interest for application of these bacteriocins to improve safety of salami during its manufacture. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/58841-2 - Bacterias laticas produtoras de bacteriocinas em salame: isolamento, caracterizacao, encapsulacao e aplicacao no controle de listeria monocytogenes em salame
• Beneficiário: Matheus de Souza Barbosa
• Modalidade de apoio: Bolsas no Brasil - Doutorado