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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Temporal analysis of prostaglandin F2 alpha receptor, caspase 3, and cyclooxygenase 2 messenger RNA expression and prostaglandin F2 alpha receptor and cyclooxygenase 2 protein expression in endometrial tissue from multiparous Nelore (Bos taurus indicus) cows treated with cloprostenol sodium during puerperium

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Autor(es):
de Moraes, Carolina N. [1] ; Maia, Leandro [1] ; de Lima, Paula F. [2] ; Dias, Marianne C. [1] ; Raposo-Ferreira, Talita M. M. [3] ; Sudano, Mateus J. [4] ; Junior, Jose B. [2] ; Oba, Eunice [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Dept Anim Reprod & Radiol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Biosci Inst, Dept Pharmacol, Botucatu, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Clin Med, Jaboticabal, SP - Brazil
[4] Fed Univ Pampa UNIPAMPA, Lab Genet & Anim Breeding, Uruguaiana, RS - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Theriogenology; v. 83, n. 2, p. 276-284, JAN 15 2015.
Citações Web of Science: 2
Resumo

The use of cloprostenol sodium in puerperium is questionable, as both favorable and unfavorable responses during the uterine involution process have been reported in the literature. This study is based on the hypothesis that cloprostenol sodium promotes modifications in the prostaglandin F2 alpha receptor (FP), caspase 3 (CASP-3), and cyclooxygenase 2 (COX-2) mRNA expression that may favor the process of postpartum uterine involution in multiparous Nelore (Bos taurus indicus) females. Additionally, we aimed to describe the presence and immunolocalization of the FP and COX-2 protein in endometrial tissue at different postpartum time points in these females. Multiparous Nelore cows (n = 24) were treated with cloprostenol sodium (n = 12) or saline solution (n = 12) on postpartum Days 1 and 4 (Day 0 = birth), and endometrial biopsies were performed with a Yomann biopsy instrument and collected on Days I, 7, 14, 28, and 42 postpartum. The mRNA expression from samples on the Days 1, 7,14, 28, and 42 and the protein expression from samples on the Days 1, 14, 28, and 42 were then analyzed. The treated cows had altered FP and CASP-3 mRNA expression, and FP and COX-2 protein were observed in the endometrial surface epithelium, the stroma, and the glandular epithelium, with cytoplasmic immunolocalization. Although we attribute the change in CASP-3 mRNA expression to physiological phenomena, the results obtained for FP mRNA expression opens new doors for the study of hormonal protocols associated with cloprostenol sodium in the puerperium of Zebu females. (C) 2015 Elsevier Inc. All rights reserved. (AU)

Processo FAPESP: 11/15330-0 - Efeito da prostaglandina f2 alfa sobre a involução uterina e expressão gênica de receptores fp durante o puerpério de vacas nelore (bos taurus indicus)
Beneficiário:Carolina Nogueira de Moraes Maia
Linha de fomento: Bolsas no Brasil - Mestrado