Epithelial-mesenchymal transition (EMT) markers have prognostic impact in multiple primary oral squamous cell carcinoma

Autor(es)

da Silva, Sabrina Daniela ^{[1, 2, 3, 4, 5, 6, 7]} ; Morand, Gregoire B. ^{[2, 3, 4, 5, 6]} ; Alobaid, Faisal A. ^{[2, 6]} ; Hier, Michael P. ^{[2, 6]} ; Mlynarek, Alex M. ^{[2, 6]} ; Alaoui-Jamali, Moulay A. ^{[2, 3, 4, 5]} ; Kowalski, Luiz Paulo ^{[1, 7]}

Afiliação do(s) autor(es)

^[1] Natl Inst Sci & Technol Oncogen INCITO, Sao Paulo - Brazil

^[2] McGill Univ, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2 - Canada

^[3] McGill Univ, Fac Med, Dept Med, Sir Mortimer B Davis Jewish Gen Hosp, Segal Canc C, Montreal, PQ - Canada

^[4] McGill Univ, Fac Med, Dept Oncol, Sir Mortimer B Davis Jewish Gen Hosp, Segal Canc C, Montreal, PQ - Canada

^[5] McGill Univ, Fac Med, Dept Pharmacol & Therapeut, Sir Mortimer B Davis Jewish Gen Hosp, Segal Canc C, Montreal, PQ - Canada

^[6] McGill Univ, Sir Mortimer B Davis Jewish Gen Hosp, Segal Canc Ctr, Dept Otolaryngol Head & Neck Surg, Montreal, PQ H3T 1E2 - Canada

^[7] AC Camargo Canc Ctr, Dept Head & Neck Surg & Otorhinolaryngol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL & EXPERIMENTAL METASTASIS; v. 32, n. 1, p. 55-63, JAN 2015.
• Citações Web of Science: 40

Resumo

Multiple primary tumors can occur in up to 35 % of the patients with head and neck cancer, however its clinicopathological features remain controversial. Deregulation of epithelial-mesenchymal transition (EMT) signaling has been associated with aggressive malignancies and tumor progression to metastasis in several cancer types. This study is the first to explore EMT process in multiple primary oral squamous cell carcinomas (OSCC). Immunohistochemical analysis of E-cadherin, catenin (alpha, beta, and gamma), APC, collagen IV, Ki-67, cyclin D1, and CD44 were performed in a tissue microarray containing multiple representative areas from 102 OSCC patients followed-up by at least 10 years. Results were analysed in relation to clinicopathological characteristics and survival rates in patients presenting multiple primary tumors versus patients without second primary tumors or metastatic disease. Significant association was observed among multiple OSCCs and protein expression of E-cadherin (P = 0.002), beta-catenin (P = 0.047), APC (P = 0.017), and cyclin D1 (P = 0.001) as well as between lymph nodes metastasis and Ki-67 staining (P = 0.021). OSCCs presenting vascular embolization were associated with negative beta-catenin membrane expression (P = 0.050). There was a significantly lower survival probability for patients with multiple OSCC (log-rank test, P < 0.0001), for tumors showing negative protein expression for E-cadherin (log-rank test, P = 0.003) and beta-catenin (log-rank test, P = 0.031). Stratified multivariate survival analysis revealed a prognostic interdependence of E-cadherin and beta-catenin co-downexpression in predicting the worst overall survival (log-rank test, P = 0.007). EMT markers have a predicted value for invasiveness related to multiple primary tumors in OSCC and co-downregulation of E-cadherin and beta-catenin has a significant prognostic impact in these cases. (AU)

• Processo FAPESP: 06/61039-8 - Identificacao de marcadores moleculares de progressao e prognostico em carcinomas epidermoides de boca.
• Beneficiário: Luiz Paulo Kowalski
• Modalidade de apoio: Auxílio à Pesquisa - Regular