Comparative Chromosome Painting in Six Species of Oligoryzomys (Rodentia, Sigmodontinae) and the Karyotype Evolution of the Genus

Autor(es)

Di-Nizo, Camilla Bruno ^[1] ; Ventura, Karen ^{[2, 3, 4]} ; Ferguson-Smith, Malcolm Andrew ^[3] ; Mary O'Brien, Patricia Caroline ^[3] ; Yonenaga-Yassuda, Yatiyo ^[2] ; Silva, Maria Jose de J. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Ecol & Evolucao, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo - Brazil

^[3] Univ Cambridge, Dept Vet Med, Cambridge Resource Ctr Comparat Genom, Cambridge - England

^[4] Univ Fed Itajuba, Inst Recursos Nat, Itajuba, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 2 FEB 6 2015.
• Citações Web of Science: 11

Resumo

Oligoryzomys belongs to the tribe Oryzomyini, and contains about 22 species. Diploid numbers range from 2n = 44 in Oligoryzomys sp. 2 to 2n = 72 in O. utiaritensis and phylogenetic relationships are not well defined. The high morphological convergence leads to misidentification of taxonomic entities and the species are often identified by chromosomal characters. Until now, the genus has been studied only by classical cytogenetic approaches. To understand the chromosomal evolution of Oligoryzomys, we developed chromosome probes from a female of Oligoryzomys moojeni (OMO) with 2n = 70 and hybridized to other five Oligoryzomys species. The probes painted 31 segments on O. fornesi (OFO) with 2n = 62; 32 segments on O. microtis (OMI), 2n = 64; 33 segments on O. nigripes (ONI), 2n = 62 and on O. rupestris (ORU), 2n = 46; and 34 on Oligoryzomys sp. 2 (OSP), 2n = 44. OMO probes 4 and 5 showed a syntenic association in O. fornesi, O. microtis and O. nigripes and were also presented in the same pair, although disrupted, in O. rupestris and Oligoryzomys sp. 2. Concerning O. rupestris and Oligoryzomys sp. 2, species with the lowest diploid numbers of the genus, a total of 8 probes hybridized to 11 segments on the largest pair of ORU 1 and 9 probes hybridized to 12 segments on OSP 1. Also, OMO 6 painted three segments in ORU, corresponding to the proximal segment of ORU 2q, and the whole of ORU 19 and 20. In OSP, the segment corresponding to ORU 20 was homologous to OSP 1p. OMO X showed signals of hybridization in both X and Y chromosomes. Extensive chromosomal rearrangements, that could not be detected by classical cytogenetic techniques, such as pericentric inversions or repositioning of centromeres, Robertsonian rearrangements and tandem fusions/fissions, as well as gain/activation or loss/inactivation of centromeres and telomeric sequences have driven the huge genome reshuffling in these closely related species. (AU)

• Processo FAPESP: 09/54300-0 - Estudos de citogenetica comparativa e de sistematica filogenetica em roedores da tribo akodontini (cricetidae, sigmodontinae): o que pode ser revelado na era da citogenomica?
• Beneficiário: Karen Ventura
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/04557-3 - Estudos citogenéticos e moleculares em pequenos roedores neotropicais
• Beneficiário: Maria José de Jesus Silva
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 10/03432-0 - Evolução cromossômica no gênero Oligoryzomys (Cricetidae: Sigmodontinae): estudos comparativos por meio de citogenética clássica e pintura cromossômica
• Beneficiário: Camilla Bruno Di Nizo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 11/50146-6 - Filogeografia comparada, filogenia, modelagem paleoclimática e taxonomia de répteis e anfíbios neotropicais
• Beneficiário: Miguel Trefaut Urbano Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático