Dietary Docosahexaenoic Acid and Eicosapentaenoic Acid Influence Liver Triacylglycerol and Insulin Resistance in Rats Fed a High-Fructose Diet

Autor(es)

de Castro, Gabriela Salim ^{[1, 2]} ; Deminice, Rafael ^[3] ; Cordeiro Simoes-Ambrosio, Livia Maria ^[1] ; Calder, Philip C. ^[2] ; Jordao, Alceu A. ^[1] ; Vannucchi, Helio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Internal Med, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Southampton, Human Dev & Hlth Acad Unit, Fac Med, Southampton SO16 6YD, Hants - England

^[3] Univ Estadual Londrina, Dept Phys Educ, Fac Phys Educ & Sport, BR-86057970 Londrina, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MARINE DRUGS; v. 13, n. 4, p. 1864-1881, 2015.
• Citações Web of Science: 18

Resumo

This study aimed to examine the benefits of different amounts of omega-3 (n-3) polyunsaturated fatty acids from fish oil (FO) on lipid metabolism, insulin resistance and gene expression in rats fed a high-fructose diet. Male Wistar rats were separated into two groups: Control (C, n = 6) and Fructose (Fr, n = 32), the latter receiving a diet containing 63% by weight fructose for 60 days. After this period, 24 animals from Fr group were allocated to three groups: FrFO2 (n = 8) receiving 63% fructose and 2% FO plus 5% soybean oil; FrFO5 (n = 8) receiving 63% fructose and 5% FO plus 2% soybean oil; and FrFO7 (n = 8) receiving 63% fructose and 7% FO. Animals were fed these diets for 30 days. Fructose led to an increase in liver weight, hepatic and serum triacylglycerol, serum alanine aminotransferase and HOMA1-IR index. These alterations were reversed by 5% and 7% FO. FO had a dose-dependent effect on expression of genes related to hepatic beta-oxidation (increased) and hepatic lipogenesis (decreased). The group receiving the highest FO amount had increased markers of oxidative stress. It is concluded that n-3 fatty acids may be able to reverse the adverse metabolic effects induced by a high fructose diet. (AU)

• Processo FAPESP: 11/10389-7 - Influência de ácidos graxos n-3 provenientes de óleo de peixe no metabolismo lipídico e expressão gênica de ratos com esteatose hepática não-alcoólica induzida por frutose
• Beneficiário: Helio Vannucchi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/00408-1 - Efeitos dos Ácidos Graxos N-3 Provenientes do Óleo de Peixe no Metabolismo Lipídico e Expressão Gênica de Ratos Alimentados com Dieta Rica em Frutose
• Beneficiário: Gabriela Salim Ferreira de Castro
• Modalidade de apoio: Bolsas no Brasil - Doutorado