A fast and highly sensitive method for the detection of canine distemper virus by the naked eye

Autor(es)

Basso, Caroline R. ^[1] ; Tozato, Claudia C. ^[2] ; Junior, Joao Pessoa A. ^[2] ; Pedrosa, Valber A. ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618000 Botucatu, SP - Brazil

^[2] UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANALYTICAL METHODS; v. 7, n. 6, p. 2264-2267, 2015.
• Citações Web of Science: 9

Resumo

An easy, low cost method for the detection of canine distemper virus (CDV) that requires no instrumentation and uses gold nanoparticle-labeled antibodies is presented. The proposed method shows a linear relationship between the concentration and signal in the range of 0 to 1.5 mu g L-1 with a detection limit of 0.7 ng mL(-1). The method was successfully applied to the detection of CDV in real samples of urine and the results from these studies correlate with values obtained using conventional methods. Our results offer the possibility of easy, rapid and reliable clinical diagnosis of CDV by monitoring the colorimetric changes and measuring linear surface plasmon resonance (LSPR) changes in urine samples. The rapid visual detection of the virus using a test assay may offer a simple and cost-effective alternative to highly complex and instrument-dependent commercial methods. (AU)

• Processo FAPESP: 14/05653-5 - Desenvolvimento de um biossensor integrado para monitoramento do metabolismo celular
• Beneficiário: Valber de Albuquerque Pedrosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/15666-1 - Detecção do vírus da cinomose canina por diferentes metodologias analíticas
• Beneficiário: Caroline Rodrigues Basso
• Modalidade de apoio: Bolsas no Brasil - Mestrado