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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A fast and highly sensitive method for the detection of canine distemper virus by the naked eye

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Basso, Caroline R. [1] ; Tozato, Claudia C. [2] ; Junior, Joao Pessoa A. [2] ; Pedrosa, Valber A. [1]
Total Authors: 4
[1] UNESP, Inst Biosci, Dept Chem & Biochem, BR-18618000 Botucatu, SP - Brazil
[2] UNESP, Inst Biosci, Dept Microbiol & Immunol, BR-18618000 Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ANALYTICAL METHODS; v. 7, n. 6, p. 2264-2267, 2015.
Web of Science Citations: 9

An easy, low cost method for the detection of canine distemper virus (CDV) that requires no instrumentation and uses gold nanoparticle-labeled antibodies is presented. The proposed method shows a linear relationship between the concentration and signal in the range of 0 to 1.5 mu g L-1 with a detection limit of 0.7 ng mL(-1). The method was successfully applied to the detection of CDV in real samples of urine and the results from these studies correlate with values obtained using conventional methods. Our results offer the possibility of easy, rapid and reliable clinical diagnosis of CDV by monitoring the colorimetric changes and measuring linear surface plasmon resonance (LSPR) changes in urine samples. The rapid visual detection of the virus using a test assay may offer a simple and cost-effective alternative to highly complex and instrument-dependent commercial methods. (AU)

FAPESP's process: 14/05653-5 - Development of an integrated biosensor for monitoring changes of cellular metabolism
Grantee:Valber de Albuquerque Pedrosa
Support Opportunities: Regular Research Grants
FAPESP's process: 12/15666-1 - Detection of canine distemper virus by different analytical methodologies
Grantee:Caroline Rodrigues Basso
Support Opportunities: Scholarships in Brazil - Master