The cytotoxic effects of brown Cuban propolis depend on the nemorosone content and may be mediated by mitochondrial uncoupling

Autor(es)

Pardo Andreu, Gilberto L. ^[1] ; Reis, Felippe H. Z. ^[2] ; Dalalio, Felipe M. ^[2] ; Nunez Figueredo, Yanier ^[3] ; Cuesta Rubio, Osmany ^[1] ; Uyemura, Sergio A. ^{[4, 5]} ; Curti, Carlos ^{[2, 5]} ; Alberici, Luciane C. ^{[2, 5]}

Afiliação do(s) autor(es)

^[1] Univ La Habana, Ctr Estudio Invest & Evaluac Biol, Inst Farm & Alimentos, Havana 13600 - Cuba

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Quim & Fis, Ribeirao Preto, SP - Brazil

^[3] Ctr Invest & Desarrollo Medicamentos, Havana - Cuba

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemico-Biological Interactions; v. 228, p. 28-34, FEB 25 2015.
• Citações Web of Science: 4

Resumo

Three main types of Cuban propolis directly related to their secondary metabolite composition have been identified: brown, red and yellow propolis; the former is majoritarian and is characterized by the presence of nemorosone. In this study, brown Cuban propolis extracts were found cytotoxic against HepG2 cells and primary rat hepatocytes, in close association with the nemorosone contents. In mitochondria isolated from rat liver the extracts displayed uncoupling activity, which was demonstrated by the increase in succinate-supported state 4 respiration rates, dissipation of mitochondrial membrane potential, Ca2+ release from Ca2+ -loaded mitochondria, and a marked ATP depletion. As in cells, the degree of such mitotoxic events was closely correlated to the nemorosone content. The propolis extracts that do not contain nemorosone were neither cytotoxic nor mitotoxic, except R-29, whose detrimental effect upon cells and mitochondria could be mediated by its isoflavonoids and chalcones components, well known mitochondrial uncouplers. Our results at least partly unravel the cytotoxic mechanism of Cuban propolis, particularly regarding brown propolis, and raise concerns about the toxicological implication of Cuban propolis consumption. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/17259-9 - Estudos sobre mecanismos de desacoplamento mitocondriais por ácidos graxos não-esterificados como estratégia de prevenção/tratamento da obesidade
• Beneficiário: Luciane Carla Alberici
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores