Testosterone induces leucocyte migration by NADPH oxidase-driven ROS- and COX2-dependent mechanisms

Autor(es)

Chignalia, Andreia Z. ^{[1, 2]} ; Oliveira, Maria Aparecida ^[1] ; Debbas, Victor ^[3] ; Dull, Randal O. ^[4] ; Laurindo, Francisco R. M. ^[3] ; Touyz, Rhian M. ^[5] ; Carvalho, Maria Helena C. ^[1] ; Fortes, Zuleica B. ^[1] ; Tostes, Rita C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, BR-05508 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Inst Heart, BR-01246 Sao Paulo, SP - Brazil

^[4] Univ Illinois, Coll Med, Chicago, IL 60612 - USA

^[5] Univ Glasgow, Inst Cardiovasc & Med Sci, Glasgow G12 8TA, Lanark - Scotland

• Tipo de documento: Artigo Científico
• Fonte: Clinical Science; v. 129, n. 1, p. 39-48, JUL 2015.
• Citações Web of Science: 16

Resumo

The mechanisms whereby testosterone increases cardiovascular risk are not clarified. However, oxidative stress and inflammation seem to be determinants. Herein, we sought to determine whether exogenous testosterone, at physiological levels, induces leucocyte migration, a central feature in immune and inflammatory responses and the mediating mechanisms. We hypothesized that testosterone induces leucocyte migration via NADPH oxidase (NADPHox)-driven reactive oxygen species (ROS) and cyclooxygenase (COX)-dependent mechanisms. Sixteen-week-old Wistar rats received an intraperitoneal injection (5 ml) of either testosterone (10(-7) mol/l) or saline. Rats were pre-treated with 5 ml of sodium salicylate (SS, non-selective COX inhibitor, 1.25x10(-3) mol/l, 1 h prior to testosterone or saline), flutamide (androgen receptor antagonist, 10 (5) mol/l), apocynin (NADPHox inhibitor, 3x10(-4) mol/l), N-{[}2-Cyclohexyloxy-4-nitrophenyl] methanesulfonamide (NS398, COX2 inhibitor, 10(-4) mol/l) or saline, 4 h before testosterone or saline administration. Leucocyte migration was assessed 24 h after testosterone administration by intravital microscopy of the mesenteric bed. Serum levels of testosterone were measured by radioimmunoassay. NADPHox activity was assessed in membrane fractions of the mesenteric bed by dihydroethidium (DHE) fluorescence and in isolated vascular smooth muscle cells (VSMC) by HPLC. NADPHox subunits and VCAM (vascular cell adhesion molecule) expression were determined by immunoblotting. Testosterone administration did not change serum levels of endogenous testosterone, but increased venular leucocyte migration to the adventia, NADPHox activity and expression (P<0.05). These effects were blocked by flutamide. SS inhibited testosterone-induced leucocyte migration (P<0.05). Apocynin and NS398 abolished testosterone-induced leucocyte migration and NADPHox activity (P<0.05). Testosterone induces leucocyte migration via NADPHox-and COX2-dependent mechanisms and may contribute to inflammatory processes and oxidative stress in the vasculature potentially increasing cardiovascular risk. (AU)

• Processo FAPESP: 04/15968-1 - Testosterona e estresse oxidativo em células de musculatura lisa muscular em cultura e vasos sanguíneos isolados de ratos
• Beneficiário: Andréia Zago Chignalia
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 04/13796-9 - Testosterona e modulação da geração de espécies reativas de oxigênio e nitrogênio no sistema cardiovascular
• Beneficiário: Maria Helena Catelli de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular