Significant differences in integration sites of Moloney murine leukemia virus/Moloney murine sarcoma virus retroviral vector carrying recombinant coagulation factor IX in two human cell lines

Autor(es)

Castilho-Fernandes, Andrielle ^{[1, 2, 3]} ; Fontes, Aparecida Maria ^[4] ; Abraham, Kuruvilla Joseph ^[5] ; Correa de Freitas, Marcela Cristina ^{[1, 2, 3]} ; da Rosa, Nathalia Gonsales ^{[1, 2, 6]} ; Picanco-Castro, Virginia ^{[1, 2]} ; de Sousa Russo-Carbolante, Elisa Maria ^{[1, 2, 7]} ; Covas, Dimas Tadeu ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Cell Therapy, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Reg Blood Ctr, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

^[3] Univ Sao Paulo, Dept Clin Med, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Fac Med Ribeirao Preto, Dept Genet, BR-14049900 Ribeirao Preto - Brazil

^[5] Univ Sao Paulo, Dept Pediat, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

^[6] Univ Sao Paulo, Dept Pharmaceut Sci, Fac Pharmaceut Sci Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

^[7] Univ Sao Paulo, Dept Clin Toxicol & Food Sci Anal, Fac Pharmaceut Sci Ribeirao Preto, BR-14049900 Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biotechnology Letters; v. 37, n. 5, p. 991-1001, MAY 2015.
• Citações Web of Science: 0

Resumo

Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive elements, fragile sites and target genes. In HepG2, rhFIX was had an increased preference for chromosomes 6 and 17; the median distance to the nearest CpG islands was 15,240 base pairs and 37 % of the integrations occurred in RefSeq genes. In Hek293, rhFIX had an increased preference for chromosome 5; the median distance to the nearest CpG islands was 209,100 base pairs and 74 % of the integrations occurred in RefSeq genes. The integrations in both cell lines were distant from the TSS. The integration patterns associated with this vector are different in each cell line. (AU)

• Processo FAPESP: 98/14247-6 - Center for Research on Cell-Based Therapy
• Beneficiário: Marco Antonio Zago
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 08/57877-3 - Instituto Nacional de Ciência e Tecnologia em Células-Tronco e Terapia Celular - INCTC
• Beneficiário: Roberto Passetto Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Temático