DNA Methylation Changes Induced by a High-Fat Diet and Fish Oil Supplementation in the Skeletal Muscle of Mice

Autor(es)

Amaral, Catia L. ^{[1, 2]} ; Crisma, Amanda R. ^[1] ; Masi, Laureane N. ^[1] ; Martins, Amanda R. ^[1] ; Hirabara, Sandro M. ^[3] ; Curi, Rui ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508000 Sao Paulo, SP - Brazil

^[2] State Univ Goias, Anapolis - Brazil

^[3] Univ Cruzeiro Sul, Inst Phys Act Sci & Sports, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Nutrigenetics and Nutrigenomics; v. 7, n. 4-6, p. 314-326, 2014.
• Citações Web of Science: 8

Resumo

Background/Aims: To investigate the global changes in DNA methylation and methylation of the promoter region of the peroxisome proliferator-activated receptor gamma transcript variant 2 (Pparg2) gene resulting from a high-fat diet (HFD) and/or fish oil supplementation. Methods: Fish oil, rich in omega-3 polyunsaturated fatty acids, or water was orally administered to male mice for 12 weeks. After the first 4 weeks, the animals were fed a control diet or an HFD until the end of the experimental protocol, when the epididymal fat, gastrocnemius muscle and liver were excised. Results: Pparg2 mRNA expression was upregulated by obesity and downregulated by fish oil supplementation in the liver. In the gastrocnemius muscle, diet-induced obesity increased global DNA methylation. Fish oil prevented the decrease in Pparg2 promoter methylation induced by obesity in the gastrocnemius muscle. Regardless of the diet given, fish oil supplementation increased Pparg2 promoter methylation at CpG-263 in muscle and adipose tissue. Conclusion: HFD and fish oil modified global and Pparg2 promoter DNA methylation in a tissue-specific manner. Fish oil supplementation attenuated body weight gain, abolished the increase in Pparg2 expression in the liver and prevented the decrease in Pparg2 promoter methylation in the muscle induced by the HFD. (C) 2015 S. Karger AG, Basel (AU)

• Processo FAPESP: 10/07596-8 - Avaliação das alterações de Metilação do DNA na obesidade induzida por dieta hiperlipídica e o possível efeito modulador dos ácidos graxos ômega-3 in vivo
• Beneficiário: Cátia Lira Do Amaral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado