| Texto completo | |
| Autor(es): Mostrar menos - |
Robertoni, Fabola S. Z.
[1]
;
Olivo, Clarice R.
[1]
;
Lourenco, Juliana D.
[1]
;
Goncalves, Natalia G.
[2]
;
Velosa, Ana Paula P.
[1]
;
Lin, Chin J.
[2]
;
Flo, Claudia M.
[1]
;
Saraiva-Romanholo, Beatriz M.
[1, 3]
;
Sasaki, Sergio D.
[4]
;
Martins, Milton A.
[1]
;
Teodoro, Walcy R.
[1]
;
Lopes, Fernanda Degobbi T. Q. S.
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med, Dept Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Phatol, Sao Paulo - Brazil
[3] Univ City Sao Paulo, Sao Paulo - Brazil
[4] UFABC, Ctr Nat & Human Sci, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS One; v. 10, n. 6 JUN 8 2015. |
| Citações Web of Science: | 7 |
| Resumo | |
To describe the progression of parenchymal remodeling and metalloproteinases gene expression in earlier stages of emphysema, mice received porcine pancreatic elastase (PPE) instillation and Control groups received saline solution. After PPE instillation (1, 3, 6 hours, 3 and 21 days) we measured the mean linear intercept, the volume proportion of types I and III collagen, elastin, fibrillin and the MMP-1, -8, -12 and -13 gene expression. We observed an initial decrease in type I (at the 3rd day) and type III collagen (from the 6th hour until the 3rd day), in posterior time points in which we detected increased gene expression for MMP-8 and -13 in PPE groups. After 21 days, the type III collagen fibers increased and the type I collagen values returned to similar values compared to control groups. The MMP-12 gene expression was increased in earlier times (3 and 6 hours) to which we detected a reduced proportion of elastin (3 days) in PPE groups, reinforcing the already established importance of MMP-12 in the breakdown of ECM. Such findings will be useful to better elucidate the alterations in ECM components and the importance of not only metalloelastase but also collagenases in earlier emphysema stages, providing new clues to novel therapeutic targets. (AU) | |
| Processo FAPESP: | 12/02957-8 - Estudo das fibras constituintes da matriz extracelular e da expressão de metaloproteases durante o desenvolvimento do enfisema pulmonar induzido por elastase em camundongos |
| Beneficiário: | Fabíola Santos Zambon Robertoni |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 11/08584-6 - Estudo das fibras constituintes da matriz extracelular e da expressão de metaloproteases durante o desenvolvimento do enfisema pulmonar induzido por elastase em camundongos |
| Beneficiário: | Fernanda Degobbi Tenorio Quirino dos Santos Lopes |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |