| Full text | |
| Author(s): Show less - |
Robertoni, Fabola S. Z.
[1]
;
Olivo, Clarice R.
[1]
;
Lourenco, Juliana D.
[1]
;
Goncalves, Natalia G.
[2]
;
Velosa, Ana Paula P.
[1]
;
Lin, Chin J.
[2]
;
Flo, Claudia M.
[1]
;
Saraiva-Romanholo, Beatriz M.
[1, 3]
;
Sasaki, Sergio D.
[4]
;
Martins, Milton A.
[1]
;
Teodoro, Walcy R.
[1]
;
Lopes, Fernanda Degobbi T. Q. S.
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Sao Paulo, Sch Med, Dept Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Phatol, Sao Paulo - Brazil
[3] Univ City Sao Paulo, Sao Paulo - Brazil
[4] UFABC, Ctr Nat & Human Sci, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | PLoS One; v. 10, n. 6 JUN 8 2015. |
| Web of Science Citations: | 7 |
| Abstract | |
To describe the progression of parenchymal remodeling and metalloproteinases gene expression in earlier stages of emphysema, mice received porcine pancreatic elastase (PPE) instillation and Control groups received saline solution. After PPE instillation (1, 3, 6 hours, 3 and 21 days) we measured the mean linear intercept, the volume proportion of types I and III collagen, elastin, fibrillin and the MMP-1, -8, -12 and -13 gene expression. We observed an initial decrease in type I (at the 3rd day) and type III collagen (from the 6th hour until the 3rd day), in posterior time points in which we detected increased gene expression for MMP-8 and -13 in PPE groups. After 21 days, the type III collagen fibers increased and the type I collagen values returned to similar values compared to control groups. The MMP-12 gene expression was increased in earlier times (3 and 6 hours) to which we detected a reduced proportion of elastin (3 days) in PPE groups, reinforcing the already established importance of MMP-12 in the breakdown of ECM. Such findings will be useful to better elucidate the alterations in ECM components and the importance of not only metalloelastase but also collagenases in earlier emphysema stages, providing new clues to novel therapeutic targets. (AU) | |
| FAPESP's process: | 12/02957-8 - STUDY OF EXTRACELLULAR MATRIX FIBER CONSTITUENTS AND EXPRESSION OF METALLOPROTEASES DURING EMPHYSEMA DEVELOPMENT INDUCED BY ELASTASE IN MICE |
| Grantee: | Fabíola Santos Zambon Robertoni |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/08584-6 - Study of extracelular matrix fiber constituints and the expression of metaloproteases during emphysema development induced by elastase in mice |
| Grantee: | Fernanda Degobbi Tenorio Quirino dos Santos Lopes |
| Support Opportunities: | Regular Research Grants |