Small RNAs in metastatic and non-metastatic oral squamous cell carcinoma

Autor(es)

Severino, Patricia ^[1] ; Oliveira, Liliane Santana ^[1] ; Andreghetto, Flavia Maziero ^[1] ; Torres, Natalia ^[1] ; Curioni, Otavio ^[2] ; Cury, Patricia Maluf ^[3] ; Toporcov, Tatiana Natasha ^[4] ; Paschoal, Alexandre Rossi ^[5] ; Durham, Alan Mitchell ^[6]

Afiliação do(s) autor(es)

^[1] Hosp Israelita Albert Einstein, Albert Einstein Res & Educ Inst, Sao Paulo, SP - Brazil

^[2] Hosp Heliopolis, Dept Cirurgia & Otorrinolaringol, Sao Paulo, SP - Brazil

^[3] Fac Ceres, Sao Jose Do Rio Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, Sao Paulo, SP - Brazil

^[5] Fed Univ Technol, Curitiba, Parana - Brazil

^[6] Univ Sao Paulo, Inst Matemat & Estatist, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MEDICAL GENOMICS; v. 8, JUN 24 2015.
• Citações Web of Science: 18

Resumo

Background: Small non-coding regulatory RNAs control cellular functions at the transcriptional and post-transcriptional levels. Oral squamous cell carcinoma is among the leading cancers in the world and the presence of cervical lymph node metastases is currently its strongest prognostic factor. In this work we aimed at finding small RNAs expressed in oral squamous cell carcinoma that could be associated with the presence of lymph node metastasis. Methods: Small RNA libraries from metastatic and non-metastatic oral squamous cell carcinomas were sequenced for the identification and quantification of known small RNAs. Selected markers were validated in plasma samples. Additionally, we used in silico analysis to investigate possible new molecules, not previously described, involved in the metastatic process. Results: Global expression patterns were not associated with cervical metastases. MiR-21, miR-203 and miR-205 were highly expressed throughout samples, in agreement with their role in epithelial cell biology, but disagreeing with studies correlating these molecules with cancer invasion. Eighteen microRNAs, but no other small RNA class, varied consistently between metastatic and non-metastatic samples. Nine of these microRNAs had been previously detected in human plasma, eight of which presented consistent results between tissue and plasma samples. MiR-31 and miR-130b, known to inhibit several steps in the metastatic process, were over-expressed in non-metastatic samples and the expression of miR-130b was confirmed in plasma of patients showing no metastasis. MiR-181 and miR-296 were detected in metastatic tumors and the expression of miR-296 was confirmed in plasma of patients presenting metastasis. A novel microRNA-like molecule was also associated with non-metastatic samples, potentially targeting cell-signaling mechanisms. Conclusions: We corroborate literature data on the role of small RNAs in cancer metastasis and suggest the detection of microRNAs as a tool that may assist in the evaluation of oral squamous cell carcinoma metastatic potential. (AU)

• Processo FAPESP: 10/51168-0 - Fatores ambientais, clínicos, histopatológicos e moleculares associados ao desenvolvimento e ao prognóstico de carcinomas epidermoides de cabeça e pescoço
• Beneficiário: Eloiza Helena Tajara da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático