Protective Immunity and Reduced Renal Colonization Induced by Vaccines Containing Recombinant Leptospira interrogans Outer Membrane Proteins and Flagellin Adjuvant

Autor(es)

Monaris, D. ^{[1, 2]} ; Sbrogio-Almeida, M. E. ^[3] ; Dib, C. C. ^[4] ; Canhamero, T. A. ^[5] ; Souza, G. O. ^[1] ; Vasconcellos, S. A. ^[1] ; Ferreira, L. C. S. ^[6] ; Abreu, P. A. E. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Sao Paulo, SP - Brazil

^[2] Inst Butantan, Lab Bacteriol, Sao Paulo, SP - Brazil

^[3] Inst Butantan, Ctr Biotecnol, Sao Paulo, SP - Brazil

^[4] Inst Biol, Lab TB, Sao Paulo, SP - Brazil

^[5] Inst Butantan, Lab Imunogenet, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Lab Desenvolvimento Vacinas, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical and Vaccine Immunology; v. 22, n. 8, p. 965-973, AUG 2015.
• Citações Web of Science: 13

Resumo

Leptospirosis is a global zoonotic disease caused by different Leptospira species, such as Leptospira interrogans, that colonize the renal tubules of wild and domestic animals. Thus far, attempts to develop effective leptospirosis vaccines, both for humans and animals, have failed to induce immune responses capable of conferring protection and simultaneously preventing renal colonization. In this study, we evaluated the protective immunity induced by subunit vaccines containing seven different recombinant Leptospira interrogans outer membrane proteins, including the carboxy-terminal portion of the immunoglobulinlike protein A (LigA(C)) and six novel antigens, combined with aluminum hydroxide (alum) or Salmonella flagellin (FliC) as adjuvants. Hamsters vaccinated with the different formulations elicited high antigen-specific antibody titers. Immunization with LigA(C), either with alum or flagellin, conferred protective immunity but did not prevent renal colonization. Similarly, animals immunized with LigA(C) or LigA(C) coadministered with six leptospiral proteins with alum adjuvant conferred protection but did not reduce renal colonization. In contrast, immunizing animals with the pool of seven antigens in combination with flagellin conferred protection and significantly reduced renal colonization by the pathogen. The present study emphasizes the relevance of antigen composition and added adjuvant in the efficacy of antileptospirosis subunit vaccines and shows the complex relationship between immune responses and renal colonization by the pathogen. (AU)

• Processo FAPESP: 10/20525-2 - Avaliação do potencial imunogênico e vacinal das flagelinas de Leptospira interrogans sorovar Copenhageni.
• Beneficiário: Denize Monaris
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/51365-0 - Contribuição ao conhecimento de Leptospira interrogans serovar Copenhageni utilizando a informação genômica
• Beneficiário: Paulo Lee Ho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/54701-6 - Desenvolvimento de um vetor para expressão de lipoproteínas de Leptospira interrogans em Escherichia coli
• Beneficiário: Patricia Antonia Estima Abreu de Aniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular