Studies of the binding of ficolin-2 and ficolin-3 from the complement lectin pathway to Leptospira biflexa, Pasteurella pneumotropica and Diarrheagenic Escherichia coli

Autor(es)

Sahagun-Ruiz, Alfredo ^[1] ; Dantas Breda, Leandro Carvalho ^[2] ; Castiblanco Valencia, Monica Marcela ^[2] ; Elias, Waldir P. ^[3] ; Munthe-Fog, Lea ^[4] ; Garred, Peter ^[4] ; Barbosa, Angela Silva ^[3] ; Isaac, Lourdes ^[2]

Afiliação do(s) autor(es)

^[1] Univ Nacl Autonoma Mexico, Dept Microbiol & Inmunol, Fac Med Vet & Zootecnia, Mexico City 04510, DF - Mexico

^[2] Univ Sao Paulo, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo, SP - Brazil

^[3] Inst Butantan, Lab Bacteriol, Sao Paulo - Brazil

^[4] Univ Copenhagen, Rigshosp, Fac Hlth & Med Sci, Lab Mol Med, Dept Clin Immunol, Sect 7631, DK-2100 Copenhagen - Denmark

• Tipo de documento: Artigo Científico
• Fonte: Immunobiology; v. 220, n. 10, p. 1177-1185, OCT 2015.
• Citações Web of Science: 8

Resumo

Ficolins recognize pathogen associated molecular patterns and activate the lectin pathway of complement system. However, our knowledge regarding pathogen recognition of human ficolins is still limited. We therefore set out to explore and investigate the possible interactions of the two main serum ficolins, ficolin-2 and ficolin-3 with different Gram-negative bacteria. We used recombinant ficolin molecules and normal human serum, which were detected with anti-ficolin monoclonal antibodies. In addition we investigated the capacity of these pathogens to activate the lectin pathway of complement system. We show for the first time that human ficolin-2 recognizes the nonpathogenic spirochete Leptospira biflexa serovar Patoc, but not the pathogenic Leptospira interrogans serovar Kennewicki strain Fromm. Additionally, human ficolin-2 and ficolin-3 recognize pathogenic Pasteurella pneumotropica, enteropathogenic Escherichia coli (EPEC) serotype O111ab:H2 and enteroaggregative E. coli (EAEC) serogroup O71 but not four enterohemorrhagic E. coli, three EPEC, three EAEC and two nonpathogenic E. coli strains (DH5 alpha and HB101). The lectin pathway was activated by Pasteurelia pneumotropica, EPEC O111ab:H2 and EAEC 071 after incubation with Clq depleted human serum. In conclusion, this study provide novel insight in the binding and complement activating capacity of the lectin pathway initiation molecules ficolin-2 and ficolin-3 towards relevant Gram-negative pathogens of pathophysiological relevance. (C) 2015 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 10/50043-0 - Sistema complemento e patogenicidade de leptospiras: mecanismos de ativação e escape identificação de ligantes bacterianos, caracterização de proteases e estabelecimento de modelo murino in vivo
• Beneficiário: Lourdes Isaac
• Modalidade de apoio: Auxílio à Pesquisa - Temático