Cross-linking proteins by laccase: Effects on the droplet size and rheology of emulsions stabilized by sodium caseinate

Autor(es)

Sato, A. C. K. ^[1] ; Perrechil, F. A. ^[2] ; Costa, A. A. S. ^[1] ; Santana, R. C. ^[3] ; Cunha, R. L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Dept Food Engn, Sch Food Engn, BR-13083862 Sao Paulo - Brazil

^[2] Fed Univ Sao Paulo UNIFESP, Inst Environm Chem & Pharmaceut Sci, BR-09913030 Diadema, SP - Brazil

^[3] Univ Fed Espirito Santo, Ctr Univ Norte Espirito Santo CEUNES, Dept Engn Tecnol, BR-29932540 Sao Mateus, ES - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 75, p. 244-251, SEP 2015.
• Citações Web of Science: 10

Resumo

The aim of this work was to evaluate the influence of laccase and ferulic acid on the characteristics of oil-in-water emulsions stabilized by sodium caseinate at different pH (3,5 and 7). Emulsions were prepared by high pressure homogenization of soybean oil with sodium caseinate solution containing varied concentrations of laccase (0, 1 and 5 mg/mL) and ferulic acid (5 and 10 mM). Laccase treatment and pH exerted a strong influence on the properties with a consequent effect on stability, structure and rheology of emulsions stabilized by Na-caseinate. At pH 7, O/W emulsions were kinetically stable due to the negative protein charge which enabled electrostatic repulsion between oil droplets resulting in an emulsion with small droplet size, low viscosity, pseudoplasticity and viscoelastic properties. The laccase treatment led to emulsions showing shear-thinning behavior as a result of a more structured system. O/W emulsions at pH 5 and 3 showed phase separation due to the proximity to protein pI, but the laccase treatment improved their stability of emulsions especially at pH 3. At pH 3, the addition of ferulic acid and laccase produced emulsions with larger droplet size but with narrower droplet size distribution, increased viscosity, pseudoplasticity and viscoelastic properties (gel-like behavior). Comparing laccase treatments, the combined addition of laccase and ferulic acid generally produced emulsions with lower stability (pH 5), larger droplet size (pH 3, 5 and 7) and higher pseudoplasticity (pH 5 and 7) than emulsion with only ferulic acid. The results suggested that the cross-linking of proteins by laccase and ferulic acid improved protein emulsifying properties by changing functional mechanisms of the protein on emulsion structure and rheology, showing that sodium caseinate can be successfully used in acid products when treated with laccase. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/54137-1 - EMUS: aquisição de um analisador de distribuição de tamanho de partículas, um medidor de potencial zeta, um spray dryer e um sistema de cromatografia líquida de ultra alta pressão / espectrômetro de massa
• Beneficiário: Miriam Dupas Hubinger
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 09/51456-9 - Avaliação da degradação de cápsulas alimentícias e liberação de compostos frente a condições bioquímicas e mecânicas do sistema gastrointestinal in vitro
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado