Human gammaherpesviruses viraemia in HIV infected patients

Autor(es)

Ferraz da Silva, Ana Paula ^[1] ; Giron, Leila Bertoni ^[1] ; da Silva, Suzane Ramos ^[1] ; Barbosa, Alexandre Naime ^[2] ; Monteiro de Barros Almeida, Ricardo Augusto ^[2] ; de Oliveira, Deilson Elgui ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Pathol, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Botucatu Med Sch, Trop Dis & Diagnost Imaging Dept, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Biotechnol Inst IBTEC, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Clinical Pathology; v. 68, n. 9, p. 726-732, SEP 2015.
• Citações Web of Science: 2

Resumo

Background The Epstein-Barr virus (EBV) and Kaposi's sarcoma associated herpesvirus (KSHV) are consistently associated with lymphoproliferative diseases and cancers in humans, notably in patients with HIV. Aims Our aim was to evaluate whether EBV and/or KSHV viral loads regularly assessed in peripheral blood mononuclear cells (PBMC) correlate with clinical or laboratorial parameters retrieved for patients living with HIV. Methods This was a longitudinal study with a cohort of 157 HIV positive patients attending an academic HIV outpatient clinic in Sao Paulo State, Brazil. For each patient, up to four blood samples were collected over a 1 year clinical follow-up: on enrolment into the study, and after 4, 8 and 12 months. Total DNA was extracted from PBMC, and EBV and KSHV viral loads were assessed by real time quantitative PCR. Results Higher viral loads for EBV were significantly associated with high HIV viraemia, a greater number of circulating T CD8+ cells and lack of virological response to the antiretroviral treatment. KSHV viral load was undetectable in virtually all samples. Conclusions EBV viral load in PBMC correlated with the number of circulating T CD8+ lymphocytes and the response to the antiretroviral therapy in HIV infected patients. In contrast, KSHV was undetectable in PBMC, presumably an effect of the antiretroviral treatment. Therefore, either KSHV infection in the population studied was absent or viral load in PBMC was beyond the analytical limit of the assay. (AU)

• Processo FAPESP: 06/03643-6 - Estudo prospectivo da infecção pelo herpesvírus associado ao sarcoma de kaposi/ Herpesvirus Humano tipo 8 (KSHV/HHV-8) em pacientes portadores do vírus da imunodeficiência humana (HIV)
• Beneficiário: Deilson Elgui de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/06033-7 - Pesquisa de gamaherpesvírus em pacientes portadores do virus da imunodeficiência humana.
• Beneficiário: Ana Paula Ferraz da Silva Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/54927-0 - Carga viral e genotipagem do virus de epstein-barr (ebv) e analise da frequencia das variantes do gene viral bnlf-1 em individuos portadores do virus da imunodeficiencia humana 1 (hiv-1)
• Beneficiário: Leila Bertoni Giron
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica