Mast cells and histamine play an important role in edema and leukocyte recruitment induced by Potamotrygon motoro stingray venom in mice

Autor(es)

Kimura, Louise F. ^[1] ; Prezotto-Neto, Jose Pedro ^[1] ; Tavora, Bianca C. L. F. ^[1] ; Faquim-Mauro, Eliana L. ^[1] ; Pereira, Nicole A. ^[1] ; Antoniazzi, Malta M. ^[2] ; Jared, Simone G. S. ^[2] ; Teixeira, Catarina F. P. ^[3] ; Santoro, Marcelo L. ^[4] ; Barbaro, Katia C. ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo, SP - Brazil

^[2] Butantan Inst, Cell Biol Lab, BR-05503900 Sao Paulo, SP - Brazil

^[3] Butantan Inst, Pharmacol Lab, BR-05503900 Sao Paulo, SP - Brazil

^[4] Butantan Inst, Lab Pathophysiol, BR-05503900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 103, p. 65-73, SEP 1 2015.
• Citações Web of Science: 6

Resumo

This work aimed to investigate mechanisms underlying the inflammatory response caused by Potamotrygon motoro stingray venom (PmV) in mouse paws. Pre-treatment of animals with a mast cell degranulation inhibitor (cromolyn) diminished edema (62% of inhibition) and leukocyte influx into the site of PmV injection. Promethazine (histamine type 1 receptor antagonist) or thioperamide (histamine type 3 and 4 receptor antagonist) also decreased edema (up to 30%) and leukocyte numbers, mainly neutrophils (40-50 %). Cimetidine (histamine type 2 receptor antagonist) had no effect on PmV-induced inflammation. In the RBL-2H3 lineage of mast cells, PmV caused proper cell activation, in a dose-dependent manner, with release of PGD(2) and PGE(2). In addition, the role of COXs products on PmV inflammatory response was evaluated. Indomethacin (COX-1/COX-2 inhibitor) or etoricoxib (COX-2 inhibitor) partially diminished edema (around 20%) in PmV-injected mice. Indomethacin, but not etoricoxib, modulated neutrophil influx into the site of venom injection. In conclusion, mast cell degranulation and histamine, besides COXs products, play an important role in PmV-induced reaction. Since PmV mechanism of action remains unknown, hindering accurate treatment, clinical studies can be performed to validate the prescription of antihistaminic drugs, besides NSAIDs, to patients injured by freshwater stingrays. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/55272-4 - Soroneutralizacao e inibicao farmacologica de atividades toxicas dos venenos de raias do genero potamotrygon.
• Beneficiário: Katia Cristina Barbaro Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/00166-3 - Participação de mastócitos e da histamina em eventos inflamatórios induzidos pelo veneno da raia Potamotrygon motoro em modelo murino
• Beneficiário: Katia Cristina Barbaro Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular