Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue

Autor(es):Mostrar menos -

Santos, Carlos F. ^[1] ; Morandini, Ana C. ^[1] ; Dionisio, Thiago J. ^[1] ; Faria, Flavio A. ^[1] ; Lima, Marta C. ^[1] ; Figueiredo, Caio M. ^[1] ; Colombini-Ishikiriama, Bella L. ^[1] ; Sipert, Carla R. ^[2] ; Maciel, Rubens P. ^[1] ; Akashi, Ana P. ^[1] ; Souza, Gabriela P. ^[1] ; Garlet, Gustavo P. ^[1] ; Rodini, Camila O. ^[1] ; Amaral, Sandra L. ^[3] ; Becari, Christiane ^[4] ; Salgado, Maria C. ^[4] ; Oliveira, Eduardo B. ^[4] ; Matus, Isaac ^[5] ; Didier, Daniela N. ^[5] ; Greene, Andrew S. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo - Brazil

^[3] Sao Paulo State Univ, Fac Sci, Dept Phys Educ, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med Ribeirao Preto, Sao Paulo - Brazil

^[5] Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 - USA

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 8 AUG 5 2015.
• Citações Web of Science: 5

Resumo

The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression were found between healthy and diseased gingiva. However, in inflamed tissue the immunoreactivity was greater for the AT(1)R compared to AT(2)R in fibroblasts. When compared to healthy tissue, ACE activity was increased in human gingiva from volunteers with gingivitis. Human-gingiva homogenates generated Ang II, Ang 1-9 and Ang 1-7 when incubated with precursors. In gingiva homogenates, Ang II formation from Ang I was nearly abolished only when captopril and chymostatin were combined. Ang 1-7 formation was significantly greater when human gingiva homogenates were incubated with chymostatin alone compared to incubation without any inhibitor, only captopril, or captopril and chymostatin. In rat gingiva, RAS components were also found; their expression was not different between healthy and experimentally induced periodontitis (EP) groups. However, renin inhibition (aliskiren) and an AT(1)R antagonist (losartan) significantly blocked EP-alveolar-bone loss in rats. Collectively, these data are consistent with the hypothesis that a local RAS system is not only present but is also functional in both human and rat periodontal tissue. Furthermore, blocking AT(1)R and renin can significantly prevent periodontal bone loss induced by EP in rats. (AU)

• Processo FAPESP: 13/16113-9 - Papel da sinalização pelo receptor purinérgico P2X7 e das deacetilases de histona na resposta imune inata de fibroblastos humanos periodontais: Implicações na patogênese da periodontite
• Beneficiário: Ana Carolina Morandini Ramos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 05/60167-0 - Expressão de pró-colágeno tipo 1, MIP-1± alfa e SDF-1 alfa por fibroblastos da polpa humana estimulados por ácido lipoteicóico de Streptococcus mutans
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/53848-1 - EMU: Aquisição de dois equipamentos de grande porte (Milliplex Analyzer Xponent 3 e acessórios e 7900 HT Fast Real Time PCR System e acessórios) para a realização de pesquisas por pesquisadores de diversos departamentos da FOB-USP e de outras instituições de pesquisa
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 09/15372-5 - Interação entre o sistema renina-angiotensina e o fator de crescimento endotelial na doença periodontal induzida experimentalmente em ratos
• Beneficiário: Daniel Thomas Brozoski
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/13479-3 - Caracterizacao de um sistema renina-angiotensina local no tecido gengival de rato.
• Beneficiário: Carlos Ferreira dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Regular