Development of a novel plaque reduction neutralisation test for hantavirus infection

Autor(es)

de Padua, Michelly ^[1] ; de Souza, William Marciel ^[1] ; Lauretti, Flavio ^[1] ; Figueiredo, Luiz Tadeu Moraes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Riberao Preto, Ctr Pesquisa Virol, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 110, n. 5, p. 624-628, AUG 2015.
• Citações Web of Science: 2

Resumo

In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (PRNT) for detecting antibodies to Brazilian hantavirus. Using PRNT, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. This procedure facilitated clear visualisation of small plaques under the microscope and provided for easy and accurate plaque counting. The sera from 37 HCPS patients from the city of Ribeirao Preto, Brazil was evaluated for the Rio Mamore virus (RIOMV) using PRNT. Six samples exhibited neutralising antibodies; these antibodies exhibited a low titre. The low level of seropositive samples may be due to fewer cross-reactions between two different hantavirus species; the patients were likely infected by Araraquara virus (a virus that has not been isolated) and RIOMV was used for the test. This assay offers a new approach to evaluating and measuring neutralising antibodies produced during hantavirus infections and it can be adapted to other hantaviruses, including viruses that will be isolated in the future. (AU)

• Processo FAPESP: 11/16866-1 - Pesquisa de anticorpos neutralizantes em pacientes com Síndrome Pulmonar e Cardiovascular e roedores infectados por hantavírus
• Beneficiário: Michelly de Pádua
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/50617-6 - Estudos sobre vírus emergentes incluindo arbovirus, robovirus, vírus respiratórios e de transmissão congênita, no Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
• Beneficiário: Luiz Tadeu Moraes Figueiredo
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/05067-0 - Estudo dos mecanismos de infecção e transmissão de hantavírus Araraquara em roedor-reservatório (Necromys lasiurus)
• Beneficiário: William Marciel de Souza
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/24150-9 - Pesquisa de vírus em roedores silvestres, mosquitos e carrapatos
• Beneficiário: William Marciel de Souza
• Linha de fomento: Bolsas no Brasil - Doutorado