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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of a novel plaque reduction neutralisation test for hantavirus infection

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de Padua, Michelly [1] ; de Souza, William Marciel [1] ; Lauretti, Flavio [1] ; Figueiredo, Luiz Tadeu Moraes [1]
Total Authors: 4
[1] Univ Sao Paulo, Fac Med Riberao Preto, Ctr Pesquisa Virol, BR-14049 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 110, n. 5, p. 624-628, AUG 2015.
Web of Science Citations: 2

In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (PRNT) for detecting antibodies to Brazilian hantavirus. Using PRNT, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. This procedure facilitated clear visualisation of small plaques under the microscope and provided for easy and accurate plaque counting. The sera from 37 HCPS patients from the city of Ribeirão Preto, Brazil was evaluated for the Rio Mamoré virus (RIOMV) using PRNT. Six samples exhibited neutralising antibodies; these antibodies exhibited a low titre. The low level of seropositive samples may be due to fewer cross-reactions between two different hantavirus species; the patients were likely infected by Araraquara virus (a virus that has not been isolated) and RIOMV was used for the test. This assay offers a new approach to evaluating and measuring neutralising antibodies produced during hantavirus infections and it can be adapted to other hantaviruses, including viruses that will be isolated in the future. (AU)

FAPESP's process: 11/16866-1 - Research of neutralizing antibodies in patients with Hantavirus pulmonary and cardiovascular syndrome and rodents infected with Araraquara Hantavirus
Grantee:Michelly de Pádua
Support type: Scholarships in Brazil - Master
FAPESP's process: 08/50617-6 - Studies on emerging viruses including arbovirus, robovirus, respiratory viruses and congenital transmission, at the Centro de Pesquisa em Virologia da Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo
Grantee:Luiz Tadeu Moraes Figueiredo
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/05067-0 - Study of the mechanisms of infection and transmission of hantaviruses in rodent reservoir Araraquara (Necromys lasiurus)
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Master