Neuroprotection and immunomodulation by xenografted human mesenchymal stem cells following spinal cord ventral root avulsion

Autor(es)

Ribeiro, Thiago B. ^[1] ; Duarte, Adriana S. S. ^[1] ; Longhini, Ana Leda F. ^{[2, 1]} ; Pradella, Fernando ^[2] ; Farias, Alessandro S. ^[2] ; Luzo, Angela C. M. ^[1] ; Oliveira, Alexandre L. R. ^[3] ; Olalla Saad, Sara Teresinha ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Sangue, Hemoctr Unicamp, Hematol & Hemotherapy Ctr, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Dept Genet Evolut & Bioagents, Neuroimmunomodulat Grp, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 5, NOV 9 2015.
• Citações Web of Science: 21

Resumo

The present study investigates the effects of xenotransplantation of Adipose Tissue Mesenchymal Stem Cells (AT-MSCs) in animals after ventral root avulsion. AT-MSC has similar characteristics to bone marrow mesenchymal stem cells (BM-MSCs), such as immunomodulatory properties and expression of neurotrophic factors. In this study, Lewis rats were submitted to surgery for unilateral avulsion of the lumbar ventral roots and received 5 x 10(5) AT-MSCs via the lateral funiculus. Two weeks after cell administration, the animals were sacrificed and the moto neurons, T lymphocytes and cell defense nervous system were analyzed. An increased neuronal survival and partial preservation of synaptophysin-positive nerve terminals, related to GDNF and BDNF expression of AT-MSCs, and reduction of pro-inflammatory reaction were observed. In conclusion, AT-MSCs prevent second phase neuronal injury, since they suppressed lymphocyte, astroglia and microglia effects, which finally contributed to rat motor-neuron survival and synaptic stability of the lesioned motorneuron. Moreover, the survival of the injected AT-MSCs lasted for at least 14 days. These results indicate that neuronal survival after lesion, followed by mesenchymal stem cell (MSC) administration, might occur through cytokine release and immunomodulation, thus suggesting that AT-MSCs are promising cells for the therapy of neuronal lesions. (AU)

• Processo FAPESP: 08/57895-1 - Instituto Nacional de Ciência e Tecnologia do Sangue
• Beneficiário: Sara Teresinha Olalla Saad
• Modalidade de apoio: Auxílio à Pesquisa - Temático