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A Euploid Line of Human Embryonic Stem Cells Derived from a 43,XX,dup(9q),+12,-14,-15,-18,-21 Embryo

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Autor(es):
Siqueira Fonseca, Simone Aparecida [1, 2] ; Costas, Roberta Montero [2] ; Morato-Marques, Mariana [1, 2] ; Costa, Silvia [1] ; Alegretti, Jose Roberto [3] ; Rosenberg, Carla [1] ; Alves da Motta, Eduardo Leme [3] ; Serafini, Paulo C. [3] ; Pereira, Lygia V. [1, 2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Natl Lab Embryon Stem Cell LaNCE, Sao Paulo - Brazil
[3] Huntington Ctr Human Reprod, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 10, n. 11 NOV 5 2015.
Citações Web of Science: 0
Resumo

Aneuploid embryos diagnosed by FISH-based preimplantation genetic screening (PGS) have been shown to yield euploid lines of human embryonic stem cells (hESCs) with a relatively high frequency. Given that the diagnostic procedure is usually based on the analysis of 1-2 blastomeres of 5 to 10-cell cleavage-stage embryos, mosaicism has been a likely explanation for the phenomena. However, FISH-based PGS can have a significant rate of misdiagnosis, and therefore some of those lines may have been derived from euploid embryos misdiagnosed as aneuploid. More recently, coupling of trophectoderm (TE) biopsy at the blastocyst stage and array-CGH lead to a more informative form of PGS. Here we describe the establishment of a new line of hESCs from an embryo with a 43, XX, dup(9q), +12,-14,-15,-18,-21 chromosomal content based on array-CGH of TE biopsy. We show that, despite the complex chromosomal abnormality, the corresponding hESC line BR-6 is euploid (46, XX). Single nucleotide polymorphism analysis showed that the embryo's missing chromosomes were not duplicated in BR-6, suggesting the existence of extensive mosaicism in the TE lineage. (AU)

Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
Beneficiário:Dimas Tadeu Covas
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs