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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Euploid Line of Human Embryonic Stem Cells Derived from a 43,XX,dup(9q),+12,-14,-15,-18,-21 Embryo

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Author(s):
Siqueira Fonseca, Simone Aparecida [1, 2] ; Costas, Roberta Montero [2] ; Morato-Marques, Mariana [1, 2] ; Costa, Silvia [1] ; Alegretti, Jose Roberto [3] ; Rosenberg, Carla [1] ; Alves da Motta, Eduardo Leme [3] ; Serafini, Paulo C. [3] ; Pereira, Lygia V. [1, 2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Dept Genet & Evolutionary Biol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Natl Lab Embryon Stem Cell LaNCE, Sao Paulo - Brazil
[3] Huntington Ctr Human Reprod, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 10, n. 11 NOV 5 2015.
Web of Science Citations: 0
Abstract

Aneuploid embryos diagnosed by FISH-based preimplantation genetic screening (PGS) have been shown to yield euploid lines of human embryonic stem cells (hESCs) with a relatively high frequency. Given that the diagnostic procedure is usually based on the analysis of 1-2 blastomeres of 5 to 10-cell cleavage-stage embryos, mosaicism has been a likely explanation for the phenomena. However, FISH-based PGS can have a significant rate of misdiagnosis, and therefore some of those lines may have been derived from euploid embryos misdiagnosed as aneuploid. More recently, coupling of trophectoderm (TE) biopsy at the blastocyst stage and array-CGH lead to a more informative form of PGS. Here we describe the establishment of a new line of hESCs from an embryo with a 43, XX, dup(9q), +12,-14,-15,-18,-21 chromosomal content based on array-CGH of TE biopsy. We show that, despite the complex chromosomal abnormality, the corresponding hESC line BR-6 is euploid (46, XX). Single nucleotide polymorphism analysis showed that the embryo's missing chromosomes were not duplicated in BR-6, suggesting the existence of extensive mosaicism in the TE lineage. (AU)

FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC