Aging does not affect the ability of human monocyte-derived dendritic cells to phagocytose Candida albicans

Autor(es)

Pereira do Nascimento, Magda Paula ^[1] ; Pinke, Karen Henriette ^[1] ; Penitenti, Marcimara ^[2] ; Valerio Ikoma, Maura Rosane ^[2] ; Lara, Vanessa Soares ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Stomatol, Bauru Sch Dent, BR-17012901 Bauru, SP - Brazil

^[2] Amaral Carvalho Fdn, Flow Cytometry Lab, BR-17210080 Jau, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AGING CLINICAL AND EXPERIMENTAL RESEARCH; v. 27, n. 6, p. 785-789, DEC 2015.
• Citações Web of Science: 6

Resumo

Background Dendritic cells (DCs) are the most potent antigen-presenting cells, playing a key role in induction of both innate and adaptive immunity. Immunosenescence refers to age-associated changes in the immune system, which may be associated with susceptibility to infections and their clinical complications. The precise effects of aging on DCs in immunity to infections are not well understood. Among the common pathogenic microorganisms, the fungus Candida albicans is an important pathogen for the development of invasive infections, especially in immunocompromised individuals, as well as during aging. Aims To make a comparative in vitro evaluation of the immunomodulatory function of DCs challenged with C. albicans, by phagocytosis of the fungal cells, and determine the involvement of TLR2 and TLR4 receptors. For this purpose, DCs were generated with the use of peripheral blood monocytes from healthy young and aged subjects. Results The phagocytosis of C. albicans is developed by DCs in TLR2- and TLR4-dependent way. This mechanism is not affected by aging. Conclusion Given the important role of the DCs in responses against the fungus, it is evident that if changes in phagocytosis occurred with aging, impairment in the elderly could develop. However, the evidence that phagocytosis of this fungus by DCs is not impaired with aging, brings us to the question of which are the mechanisms truly associated with the prevalence of certain diseases in the elderly. (AU)

• Processo FAPESP: 10/15271-1 - Imunossenescência e células dendríticas: o papel dos receptores TLR2 e TLR4 na fagocitose e na produção de citocinas frente ao desafio com Candida albicans
• Beneficiário: Vanessa Soares Lara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/17889-5 - Imunossenescência e Células dendríticas: o papel dos receptores TLR2 e TLR4 na fagocitose e na produção de citocinas frente ao desafio com Candida albicans.
• Beneficiário: Magda Paula Pereira Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado