A plant proteinase inhibitor from Crataeva tapia (CrataBL) attenuates elastase-induced pulmonary inflammatory, remodeling, and mechanical alterations in mice

Autor(es):Mostrar menos -

Oliva, Leandro V. ^[1] ; Almeida-Reis, Rafael ^[1] ; Theodoro-Junior, Osmar ^[1] ; Oliveira, Bruno M. ^[1] ; Leick, Edna A. ^[1] ; Prado, Carla M. ^[2] ; Brito, Marlon V. ^[3] ; dos Santos Correia, Maria Tereza ^[4] ; Paiva, Patricia M. G. ^[4] ; Martins, Milton A. ^[1] ; Oliva, Maria Luiza V. ^[2] ; Tiberio, Iolanda F. L. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Dept Clin Med, BR-09500900 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, SP - Brazil

^[4] Univ Fed Pernambuco, Dept Bioquim, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Process Biochemistry; v. 50, n. 11, p. 1958-1965, NOV 2015.
• Citações Web of Science: 2

Resumo

Chronic obstructive pulmonary disease (COPD) can lead to chronic and obstructive bronchitis and emphysema resulting in decreased bronchial lumen size. This study evaluated the effect of CrataBL, a protein isolated from the bark of Crataeva tapia, on lung mechanics, inflammation, and remodeling after elastase-induced pulmonary alterations in mice. The use of CrataBL led to decreased mechanical alterations, alveolar septum disruption (Lm), number of macrophage and neutrophil cells in the BALF, and TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS positive cells in the airways and alveolar walls compared to the animals in the ELA group. Moreover, a reduction in MUC-5-positive cells in the airway walls was observed. In conclusion, CrataBL attenuates changes in lung mechanics, inflammation, extracellular lung remodeling, and oxidative stress responses induced by the administration of elastase and decreased the volume fraction of isoprostane, collagen, and elastic fibers in the airways and alveolar walls compared to the animals in the ELA groups. Therefore, CrataBL is a potential therapeutic tool in the treatment of COPD. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/01165-8 - Efeito do inibidor de proteinase de origem vegetal (CrataBL) na lesão pulmonar induzida pela elastase em camundongos C57Bl6
• Beneficiário: Leandro Vilela Oliva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/53766-5 - Proteínas de origem vegetal com seletividade para inibição de enzimas de mamíferos e seu papel como agente anti-inflamatório, antitrombótico, antidiabético e antitumoral
• Beneficiário: Maria Luiza Vilela Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Temático