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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A plant proteinase inhibitor from Crataeva tapia (CrataBL) attenuates elastase-induced pulmonary inflammatory, remodeling, and mechanical alterations in mice

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Oliva, Leandro V. [1] ; Almeida-Reis, Rafael [1] ; Theodoro-Junior, Osmar [1] ; Oliveira, Bruno M. [1] ; Leick, Edna A. [1] ; Prado, Carla M. [2] ; Brito, Marlon V. [3] ; dos Santos Correia, Maria Tereza [4] ; Paiva, Patricia M. G. [4] ; Martins, Milton A. [1] ; Oliva, Maria Luiza V. [2] ; Tiberio, Iolanda F. L. C. [1]
Total Authors: 12
[1] Univ Sao Paulo, Fac Med, Dept Clin Med, BR-09500900 Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Ciencias Biol, Diadema, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Bioquim, BR-04044020 Sao Paulo, SP - Brazil
[4] Univ Fed Pernambuco, Dept Bioquim, Recife, PE - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Process Biochemistry; v. 50, n. 11, p. 1958-1965, NOV 2015.
Web of Science Citations: 2

Chronic obstructive pulmonary disease (COPD) can lead to chronic and obstructive bronchitis and emphysema resulting in decreased bronchial lumen size. This study evaluated the effect of CrataBL, a protein isolated from the bark of Crataeva tapia, on lung mechanics, inflammation, and remodeling after elastase-induced pulmonary alterations in mice. The use of CrataBL led to decreased mechanical alterations, alveolar septum disruption (Lm), number of macrophage and neutrophil cells in the BALF, and TNF-alpha, MMP-9, MMP-12, TIMP-1, eNOS, and iNOS positive cells in the airways and alveolar walls compared to the animals in the ELA group. Moreover, a reduction in MUC-5-positive cells in the airway walls was observed. In conclusion, CrataBL attenuates changes in lung mechanics, inflammation, extracellular lung remodeling, and oxidative stress responses induced by the administration of elastase and decreased the volume fraction of isoprostane, collagen, and elastic fibers in the airways and alveolar walls compared to the animals in the ELA groups. Therefore, CrataBL is a potential therapeutic tool in the treatment of COPD. (C) 2015 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/01165-8 - Effect of plant proteinase inhibitor (CrataBL) in elastase-induced lung injury in C57Bl6 mice
Grantee:Leandro Vilela Oliva
Support type: Scholarships in Brazil - Master
FAPESP's process: 09/53766-5 - Proteins from plant source with selectivity for inhibition of mammalian enzymes and their role as an anti-inflammatory, antithrombotic, anti-diabetic and anti-tumor agent
Grantee:Maria Luiza Vilela Oliva
Support type: Research Projects - Thematic Grants