Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Melatonergic system-based two-gene index is prognostic in human gliomas

Texto completo
Autor(es):
Kinker, Gabriela S. [1] ; Oba-Shinjo, Sueli M. [2] ; Carvalho-Sousa, Claudia E. [1] ; Muxel, Sandra M. [1] ; Marie, Suely K. N. [2, 3] ; Markus, Regina P. [1, 3] ; Fernandes, Pedro A. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Med, Dept Neurol, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Ctr Convergence Life Sci Phys Sci & Engn Innovat, BR-05508090 Sao Paulo, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Pineal Research; v. 60, n. 1, p. 84-94, JAN 2016.
Citações Web of Science: 7
Resumo

Gliomas, the most common primary brain tumors in adults, are classified into four malignancy grades according to morphological features. Recent studies have shown that melatonin treatment induces cytotoxicity in glioma-initiating cells and reduces the invasion and migration of glioma cell lines, inhibiting the nuclear factor kappa B (NF kappa B) oncopathway. Given that C6 rat glioma cells produce melatonin, we investigated the correlation between the capacity of gliomas to synthesize/metabolize melatonin and their overall malignancy. We first characterized the melatonergic system of human gliomas cell lines with different grades of aggressiveness (HOG, T98G, and U87MG) and demonstrated that glioma-synthesized melatonin exerts an autocrine antiproliferative effect. Accordingly, the sensitivity to exogenous melatonin was higher for the most aggressive cell line, U87MG, which synthesized/accumulated less melatonin. Using The Cancer Genome Atlas RNAseq data of 351 glioma patients, we designed a predictive model of the content of melatonin in the tumor microenvironment, the ASMT:CYP1B1 index, combining the gene expression levels of melatonin synthesis and metabolism enzymes. The ASMT: CYP1B1 index negatively correlated with tumor grade, as well as with the expression of pro-proliferation and anti-apoptotic NF kappa B target genes. More importantly, the index was a grade-and histological type-independent prognostic factor. Even when considering only high-grade glioma patients, a low ASMT: CYP1B1 value, which suggests decreased melatonin and enhanced aggressiveness, was strongly associated with poor survival. Overall, our data reveal the prognostic value of the melatonergic system of gliomas and provide insights into the therapeutic role of melatonin. (AU)

Processo FAPESP: 10/52687-1 - Caracterização do papel da inter-relação adrenal-pineal no contexto do eixo imune-pineal
Beneficiário:Pedro Augusto Carlos Magno Fernandes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/13691-1 - Eixo imune-pineal: integrando a biologia do tempo em condições fisiológicas, fisiopatológicas e patológicas
Beneficiário:Regina Pekelmann Markus
Linha de fomento: Auxílio à Pesquisa - Temático