Mast Cell Proteases 6 and 7 Stimulate Angiogenesis by Inducing Endothelial Cells to Release Angiogenic Factors

Autor(es)

de Souza Junior, Devandir Antonio ^[1] ; Borges, Antonio Carlos ^[1] ; Santana, Ana Carolina ^[1] ; Oliver, Constance ^[1] ; Jamur, Maria Celia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 12 DEC 3 2015.
• Citações Web of Science: 4

Resumo

Mast cell proteases are thought to be involved with tumor progression and neo-vascularization. However, their exact role is still unclear. The present study was undertaken to further elucidate the function of specific subtypes of recombinant mouse mast cell proteases (rmMCP-6 and 7) in neo-vascularization. SVEC4-10 cells were cultured on Geltrex (R) with either rmMCP-6 or 7 and tube formation was analyzed by fluorescence microscopy and scanning electron microscopy. Additionally, the capacity of these proteases to induce the release of angiogenic factors and pro and anti-angiogenic proteins was analyzed. Both rmMCP-6 and 7 were able to stimulate tube formation. Scanning electron microscopy showed that incubation with the proteases induced SVEC4-10 cells to invade the gel matrix. However, the expression and activity of metalloproteases were not altered by incubation with the mast cell proteases. Furthermore, rmMCP-6 and rmMCP-7 were able to induce the differential release of angiogenic factors from the SVEC4-10 cells. rmMCP-7 was more efficient in stimulating tube formation and release of angiogenic factors than rmMCP-6. These results suggest that the subtypes of proteases released by mast cells may influence endothelial cells during in vivo neo-vascularization. (AU)

• Processo FAPESP: 11/10363-8 - A ação dos subtipos de proteases específicas de mastócitos sobre as células endoteliais durante a angiogênese
• Beneficiário: Devandir Antonio de Souza Junior
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/54013-0 - Correlação funcional entre mastócitos e a angiogênese tumoral
• Beneficiário: Maria Célia Jamur
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários