| Full text | |
| Author(s): |
de Souza Junior, Devandir Antonio
[1]
;
Borges, Antonio Carlos
[1]
;
Santana, Ana Carolina
[1]
;
Oliver, Constance
[1]
;
Jamur, Maria Celia
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cell & Mol Biol & Pathogen Bioagents, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | PLoS One; v. 10, n. 12 DEC 3 2015. |
| Web of Science Citations: | 4 |
| Abstract | |
Mast cell proteases are thought to be involved with tumor progression and neo-vascularization. However, their exact role is still unclear. The present study was undertaken to further elucidate the function of specific subtypes of recombinant mouse mast cell proteases (rmMCP-6 and 7) in neo-vascularization. SVEC4-10 cells were cultured on Geltrex (R) with either rmMCP-6 or 7 and tube formation was analyzed by fluorescence microscopy and scanning electron microscopy. Additionally, the capacity of these proteases to induce the release of angiogenic factors and pro and anti-angiogenic proteins was analyzed. Both rmMCP-6 and 7 were able to stimulate tube formation. Scanning electron microscopy showed that incubation with the proteases induced SVEC4-10 cells to invade the gel matrix. However, the expression and activity of metalloproteases were not altered by incubation with the mast cell proteases. Furthermore, rmMCP-6 and rmMCP-7 were able to induce the differential release of angiogenic factors from the SVEC4-10 cells. rmMCP-7 was more efficient in stimulating tube formation and release of angiogenic factors than rmMCP-6. These results suggest that the subtypes of proteases released by mast cells may influence endothelial cells during in vivo neo-vascularization. (AU) | |
| FAPESP's process: | 11/10363-8 - The action of specific proteases of subtypes of mast cells on endothelial cells during angiogenesis |
| Grantee: | Devandir Antonio de Souza Junior |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 09/54013-0 - Functional correlation between mast cells and tumor angiogenesis |
| Grantee: | Maria Célia Jamur |
| Support Opportunities: | Multi-user Equipment Program |