Angiogenesis is the formation of new blood vessels from pre-existing vasculature, and the vascular endothelial growth factor (VEGF) is a key regulator in this process, being a survival factor for endothelial cells, stimulating the origin of new microvascular network. The angiogenesis process is extremely necessary for the success of transplants, regeneration and repair of damaged tissues, post-ischemic recovery and wound healing. Thus, understanding the growth intensification of new microvascular systems is critical to new therapeutic strategies. Recent studies have shown that the protein Annexin A1 (ANXA1) is involved on regulation of inflammation, apoptosis, cell growth, differentiation and also in the balance between physiological and pathological angiogenesis. In vivo investigations of our research group showed that ANXA1 can act as an inducer of physiological angiogenesis, as well as serine proteases from Bothrops whose data were noted in recent vitro studies. With these considerations, our aim is to investigate, in vivo, the process of angiogenesis after prior/current treatment with ANXA1 and serine proteases from Bothrops brazili and atrox. The effects of ANXA1 and serine proteases on the kinetics of formation of new vessels in the microcirculatory network will be investigated in experimental models of dorsal chambers implantation in Balb/c wild mice. Although some investigations identify the involvement of ANXA1 and serine proteases in the regulation of angiogenesis, the exact role in physiology is not fully understood. Thus, our studies may reveal important functions of these mediators in this process, which involves proliferation and differentiation of endothelial cells, extracellular matrix and surrounding cells.
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