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Investigation of the action of annexin a1 protein on the angiogenesis process induced by vascular endothelial growth factor: experimental models in vivo and in vitro

Grant number: 13/07487-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2013
Effective date (End): February 28, 2015
Field of knowledge:Biological Sciences - Morphology - Histology
Principal Investigator:Sonia Maria Oliani
Grantee:Jéssica Zani Lacerda
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


The angiogenesis consists in the formation of new blood vessels from the pre-existenting vasculature and the vascular endothelial growth factor (VEGF) is a key regulator in this process, being a survival factor for endothelial cells, stimulates the origin of the new microvascular network. The process of angiogenesis is extremely necessary for successful transplantation, regeneration and repair of damaged tissue recovery after ischemia and wound healing. Thus, understanding of enhancing growth of new microvascular system is essential for new therapeutic strategies. Recent studies have shown that the protein annexin A1 (ANXA1) is involved in the regulation of inflammation, apoptosis, cell growth, differentiation, and also the balance between physiological and pathological angiogenesis, and can act as a pro-angiogenic factor. With these considerations, our aim is to investigate the role of this protein in the process of angiogenesis, using experimental models in vivo and in vitro induced by VEGF. The effects of ANXA1 on the kinetics of formation of new vessels in vivo microcirculatory network, will be investigated in an experimental model of dorsal chambers implatation in Balb/c wild and ANXA1 knockout mouse. In investigations evaluate in vitro, in cell line of human umbilical vein endothelial cells (HUVEC), the effect of protein ANXA1 in the following aspects: cell viability, proliferation, migration, tube formation, adhesion molecule expression, adhesion and quantification of ANXA1 endogenous. Although some investigations have identified the involvement of ANXA1 in the regulation of angiogenesis, the exact role in the physiology is not fully understood. Thus, our studies, taken together, might reveal important functions of ANXA1 in this process that involves proliferation and differentiation of endothelial cells and extracellular matrix surrounding cells. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LACERDA, JESSICA ZANI; DREWES, CARINE CRISTIANE; OLIVEIRA MIMURA, KALLYNE KIOKO; ZANON, CAROLINE DE FREITAS; ANSARI, TAHERA; GIL, CRISTIANE DAMAS; GRECO, KARIN VICENTE; POLISELLI FARSKY, SANDRA HELENA; OLIANI, SONIA MARIA. Annexin A1(2-26) Treatment Improves Skin Heterologous Transplantation by Modulating Inflammation and Angiogenesis Processes. FRONTIERS IN PHARMACOLOGY, v. 9, . (13/07487-2, 12/13041-4, 12/21603-2, 16/02012-4, 10/19802-1, 14/07328-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LACERDA, Jéssica Zani. Investigação da ação da proteína anexina A1 sobre o processo de angiogênese induzido pelo fator de crescimento do endotélio vascular: modelos experimentais in vivo e in vitro. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

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