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Biochemical and functional isolation and characterization of a metalloproteases inhibitor present in the snake serum of Bothrops alternatus

Grant number: 12/02734-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2012
Effective date (End): January 31, 2014
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Suely Vilela
Grantee:Tatiana Zapata Palacio
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Snake venom is a complex mixture of toxins and enzymes showing several activities on the biological system. They are rich in proteolitic enzymes with specific biological activities, such as hemorrhagic, coagulation disorders, necroses and inflammation. The just mentioned proteolitic enzymes, wich are named serine proteases and metalloproteases, affect the hemostatic system by degrading coagulation factors, activating specific factors in coagulation cascade acting on the fibrinogenolysis and/or fibrinolysis or degrading proteins in the endothelial surface. The presence of components in animal plasma, which confer resistance to several snake venoms have been reported. The goal of this work is to isolate and characterize biochemical and functionally a protease inhibitor present in the plasma of Bothrops alternatus, and to understand the action neutralizing mechanism of the proteases present in Bothrops venom (serine proteases and metalloproteases of B. atrox and B. jararacussu). Therefore, new information related to these inhibitors will be known, in the tentative of a better understanding of their use as a new therapeutic model with antivenom action in the neutralization of different kind of proteases, in the supplementation of the conventional serum therapy and in the molecular interaction with these toxins in order to comprehend the action mechanisms involving these multifunctional enzymes.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PALACIO, TATIANA Z.; SANTOS-FILHO, NORIVAL A.; ROSA, JOSE CESAR; FERREIRA JUNIOR, RUI S.; BARRAVIERA, BENEDITO; SAMPAIO, SUELY V.. Isolation and characterization of a novel metalloprotease inhibitor from Bothrops alternatus snake serum. International Journal of Biological Macromolecules, v. 98, p. 436-446, . (12/02734-9, 11/23236-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PALACIO, Tatiana Zapata. Isolation and biochemical and functional characterization of a metalloprotease inhibitor from Bothrops alternatus snake serum. 2014. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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