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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and characterization of a novel metalloprotease inhibitor from Bothrops alternatus snake serum

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Author(s):
Palacio, Tatiana Z. ; Santos-Filho, Norival A. ; Rosa, Jose Cesar ; Ferreira Junior, Rui S. ; Barraviera, Benedito ; Sampaio, Suely V.
Total Authors: 6
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 98, p. 436-446, MAY 2017.
Web of Science Citations: 5
Abstract

Resistance of snakes and some other animals to snake envenomation has been attributed to soluble factors present in their tissues. Here we report the isolation of a novel metalloprotease inhibitor from Bothrops alternatus snake serum (named BaltMPI) with high purity, using a four-step chromatographic method. BaltMPI has molecular weights of 60.5 and 42.4 kDa, as determined by SDS-PAGE and mass spectrometry, respectively, and pI = 5.27. The first 60 amino acids from the N-terminal region of BaltMPI, determined by Edman's degradation, showed high homology (97%) with the snake venom metalloprotease inhibitor (SVMPI) BJ46a and other SVMPIs (78-82%). The chromatographic fractions and purified BaltMPl exhibited anti-hemorrhagic activity against Batroxase and BjussuMP-I. BaltMPl was stable over wide ranges of pH (1, 5, 8, and 9) and temperature (-80, 20, 4, 60, and 100 degrees C), and suppressed the fibrinogenolytic, fibrinolytic, and azocaseinolytic activities of Batroxase. BaltMPI specifically inhibited the activity of metalloproteases, without affecting the activity of serine proteases. Together, our results suggest that BaltMPl and other SVMPIs are promising molecules for the treatment of snake envenomation, in particular that caused by Bothrops sp. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/02734-9 - Biochemical and functional isolation and characterization of a metalloproteases inhibitor present in the snake serum of Bothrops alternatus
Grantee:Tatiana Zapata Palacio
Support type: Scholarships in Brazil - Master
FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
Grantee:Suely Vilela
Support type: Research Projects - Thematic Grants