Study of peptidase activity of the B. jararaca venom and potential neutralizing serum produced at Instituto Butantan upon this activity: new aspects of Bothrops poisoning

• Grant number: 12/06677-0
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2012 - December 31, 2014
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Fernanda Calheta Vieira Portaro
• Grantee: Fernanda Calheta Vieira Portaro
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Although the ophidian poisoning overcome various other neglected tropical diseases in terms of number of occurrence and deaths, such as leishmaniasis, dengue, schistosomiasis, cholera and Chagas disease, it was only recently recognized as an important issue of public health. The genus Bothrops is considered the most important in the cases of poisoning in Brazil and, as well as on other snake, their venom is a complex mixture of constituents that act on different targets in the prey/victim. Because proteolytic enzymes from the classes of metalopeptidases and serinopeptidases (about 65 % of the composition of poison) are the major toxins from these venoms, they were chosen for the development of this research project. Although there is much information about proteic substrates for these enzymes, few data for the peptidasic activity are available. Recently in our laboratory we have been able to identify a new chymotrypsin-like angiotensin degrading activity in the venom of five Botrhops species which is only partially blocked by anti-botrhopic serum produced by the Instituto Butantan (Kuniyoshi et al., 2010). These results, describing a new peptidasic activity in bothropic venoms, opened the opportunity to amplify the studies using other biologically active peptides and who may be participating in the framework of human poisoning by these animals. To do this we will use a series of biologically active peptides and commercial site-targeted inhibitors against the serinopeptidases and metalopeptidases, the major toxins of this poison. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KODAMA, ROBERTO T.; CAJADO-CARVALHO, DANIELA; KUNIYOSHI, ALEXANDRE K.; KITANO, EDUARDO S.; TASHIMA, ALEXANDRE K.; BARNA, BARBARA F.; TAKAKURA, ANA CAROLINA; SERRANO, SOLANGE M. T.; DIAS-DA-SILVA, WILMAR; TAMBOURGI, DENISE V.; et al. New proline-rich oligopeptides from the venom of African adders: Insights into the hypotensive effect of the venoms. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1850, n. 6, p. 1180-1187, JUN 2015. (12/06677-0)

CARVALHO, DANIELA CAJADO; KUNIYOSHI, ALEXANDRE K.; KODAMA, ROBERTO T.; OLIVEIRA, ANA K.; SERRANO, SOLANGE M. T.; TAMBOURGI, DENISE V.; PORTARO, FERNANDA V.. Neuropeptide Y Family-Degrading Metallopeptidases in the Tityus serrulatus Venom Partially Blocked by Commercial Antivenoms. TOXICOLOGICAL SCIENCES, v. 142, n. 2, p. 418-426, DEC 2014. (12/06677-0, 13/15343-0)

DUZZI, BRUNO; CAJADO-CARVALHO, DANIELA; KUNIYOSHI, ALEXANDRE KAZUO; KODAMA, ROBERTO TADASHI; GOZZO, FABIO CESAR; FIORAMONTE, MARIANA; TAMBOURGI, DENISE VILARINHO; PORTARO, FERNANDA VIEIRA; RIOLI, VANESSA. [des-Arg(1)]-Proctolin: A novel NEP-like enzyme inhibitor identified in Tityus serrulatus venom. Peptides, v. 80, p. 18-24, JUN 2016. (12/06677-0, 14/12976-5, 13/15343-0)

KUNIYOSHI, ALEXANDRE KAZUO; KODAMA, ROBERTO TADASHI; CAJADO-CARVALHO, DANIELA; IWAI, LEO KEI; KITANO, EDUARDO; FERNANDES DA SILVA, CRISTIANE CASTILHO; DUZZI, BRUNO; DA SILVA, WILMAR DIAS; PORTARO, FERNANDA CALHETA. Experimental antivenom against serine proteases from the Bothrops jararaca venom obtained in mice, and its comparison with the antibothropic serum from the Butantan Institute. Toxicon, v. 169, p. 59-67, NOV 2019. (15/13124-5, 13/07467-1, 13/15344-7, 12/06677-0, 15/15364-3)

KUNIYOSHI, ALEXANDRE KAZUO; KODAMA, ROBERTO TADASHI; FERREIRA MORAES, LUIS HENRIQUE; DUZZI, BRUNO; IWAI, LEO KEI; LIMA, ISMAEL FEITOSA; CAJADO-CARVALHO, DANIELA; PORTARO, FERNANDA VIEIRA. In vitro cleavage of bioactive peptides by peptidases from Bothrops jararaca venom and its neutralization by bothropic antivenom produced by Butantan Institute: Major contribution of serine peptidases. Toxicon, v. 137, p. 114-119, OCT 2017. (13/07467-1, 15/13124-5, 12/06677-0, 15/15364-3, 13/15344-7)