MHC Class I Chain-Related Gene A Polymorphisms and Linkage Disequilibrium with HLA-B and HLA-C Alleles in Ocular Toxoplasmosis

Autor(es)

Ayo, Christiane Maria ^{[1, 2]} ; da Silveira Camargo, Ana Vitoria ^{[1, 2]} ; Frederico, Fabio Batista ^{[3, 1]} ; Siqueira, Rubens Camargo ^[2] ; Previato, Mariana ^{[1, 2]} ; Antunes Murata, Fernando Henrique ^{[1, 2]} ; Silveira-Carvalho, Aparecida Perpetuo ^[2] ; Barbosa, Amanda Pires ^{[3, 1]} ; Brandao de Mattos, Cinara de Cassia ^{[1, 2]} ; de Mattos, Luiz Carlos ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fundacao Fac Reg Med Sao Jose Do Rio Preto, FAMERP Toxoplasma Res Grp, Sao Jose Do Rio Preto, SP - Brazil

^[2] Fac Med Sao Jose Rio Preto Sao Jose Rio Preto, Dept Mol Biol, Immunogenet Lab, Sao Jose Do Rio Preto, SP - Brazil

^[3] Hosp Base Sao Jose Rio Preto, Ophthalmol Outpatient Clin, Fundacao Fac Reg Med Sao Jose Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 12 DEC 16 2015.
• Citações Web of Science: 3

Resumo

This study investigated whether polymorphisms of the MICA (major histocompatibility complex class I chain-related gene A) gene are associated with eye lesions due to Toxoplasma gondii infection in a group of immunocompetent patients from southeastern Brazil. The study enrolled 297 patients with serological diagnosis of toxoplasmosis. Participants were classified into two distinct groups after conducting fundoscopic exams according to the presence (n = 148) or absence (n = 149) of ocular scars/lesions due to toxoplasmosis. The group of patients with scars/lesions was further subdivided into two groups according to the type of the ocular manifestation observed: primary (n = 120) or recurrent (n = 28). Genotyping of the MICA and HLA alleles was performed by the polymerase chain reaction-sequence specific oligonucleotide technique (PCR-SSO; One Lambda (R)) and the MICA-129 polymorphism (rs1051792) was identified by nested polymerase chain reaction (PCR-RFLP). Significant associations involving MICA polymorphisms were not found. Although the MICA{*}002 similar to HLA-B{*}35 haplotype was associated with increased risk of developing ocular toxoplasmosis (P-value = 0.04; OR = 2.20; 95% CI = 1.05-4.60), and the MICA{*}008 similar to HLA-C{*}07 haplotype was associated with protection against the development of manifestations of ocular toxoplasmosis (P-value = 0.009; OR: 0.44; 95% CI: 0.22-0.76), these associations were not statistically significant after adjusting for multiple comparisons. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population. (AU)

• Processo FAPESP: 13/15879-8 - Infecção por Toxoplasma gondii: caracterização sorológica e molecular em pacientes atendidos pelo SUS
• Beneficiário: Fernando Henrique Antunes Murata
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/25650-8 - Genes HLA de classe II (DRB1 e DQB1) como fatores de risco para a toxoplasmose ocular
• Beneficiário: Luiz Carlos de Mattos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/06580-9 - Genes KIR, seus ligantes HLA e polimorfismo do gene MICA na toxoplasmose ocular e na forma digestiva da doença de Chagas
• Beneficiário: Christiane Maria Ayo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/10050-5 - Avaliação sorológica, molecular e de imagens em pacientes com lesão ativa e ou reagudização de infecção ocular por Toxoplasma gondii: um estudo de follow-up
• Beneficiário: Mariana Previato
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 09/17540-2 - Sistemas histo-sanguíneos como fatores de risco para a toxoplasmose ocular
• Beneficiário: Luiz Carlos de Mattos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/13723-6 - MHC class I chain-related gene A polymorphisms and linkage disequilibrium with HLAB and HLA-C alleles in ocular toxoplasmosis
• Beneficiário: Cinara de Cássia Brandão
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo