Ontogenetic Variation in Biological Activities of Venoms from Hybrids between Bothrops erythromelas and Bothrops neuwiedi Snakes

Autor(es)

Santoro, Marcelo Larami ^[1] ; do Carmo, Thais ^[1] ; Lopes Cunha, Bruna Heloisa ^[1] ; Alves, Andre Fonseca ^[1] ; Zelanis, Andre ^{[2, 3]} ; de Toledo Serrano, Solange Maria ^{[2, 3]} ; Grego, Kathleen Fernandes ^[4] ; Sant'Anna, Savio Stefanini ^[4] ; Barbaro, Katia Cristina ^[5] ; Fernandes, Wilson ^[4]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Fisiopatol, Sao Paulo, SP - Brazil

^[2] Inst Butantan, Lab Especial Toxinol Aplicada, Sao Paulo, SP - Brazil

^[3] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling, Sao Paulo, SP - Brazil

^[4] Inst Butantan, Lab Herpetol, Sao Paulo, SP - Brazil

^[5] Inst Butantan, Lab Imunopatol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 10, n. 12 DEC 29 2015.
• Citações Web of Science: 5

Resumo

Lance-headed snakes are found in Central and South America, and they account for most snakebites in Brazil. The phylogeny of South American pitvipers has been reviewed, and the presence of natural and non-natural hybrids between different species of Bothrops snakes demonstrates that reproductive isolation of several species is still incomplete. The present study aimed to analyze the biological features, particularly the thrombin-like activity, of venoms from hybrids born in captivity, from the mating of a female Bothrops erythromelas and a male Bothrops neuwiedi, two species whose venoms are known to display ontogenetic variation. Proteolytic activity on azocoll and amidolytic activity on N-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA) were lowest when hybrids were 3 months old, and increased over body growth, reaching values similar to those of the father when hybrids were 12 months old. The clotting activity on plasma diminished as hybrids grew; venoms from 3- and 6-months old hybrids showed low clotting activity on fibrinogen (i.e., thrombinlike activity), like the mother venom, and such activity was detected only when hybrids were older than 1 year of age. Altogether, these results point out that venom features in hybrid snakes are genetically controlled during the ontogenetic development. Despite the presence of the thrombin-like enzyme gene(s) in hybrid snakes, they are silenced during the first six months of life. (AU)

• Processo FAPESP: 08/02996-8 - Venenos de serpentes Bothrops jararaca recém-nascidas e adultas: comparação entre suas atividades enzimáticas e biológicas, imunoneutralização, composição química e capacidade de induzir alterações hemostáticas
• Beneficiário: Marcelo Larami Santoro
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/08162-1 - Contribuição das lesões locais induzidas pelo veneno da serpente Bothrops jararaca para a gênese de distúrbios hemostáticos sistêmicos
• Beneficiário: Marcelo Larami Santoro
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/25177-0 - Envolvimento do fator de Von Willebrand nos distúrbios hemostáticos do envenenamento pela serpente Bothrops jararaca: estudo experimental
• Beneficiário: Marcelo Larami Santoro
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/15413-3 - Caracterização das atividades biológicas de venenos de filhotes de serpentes híbridas de Bothrops erythromelas e Bothrops neuwiedi
• Beneficiário: Thaís Do Carmo
• Linha de fomento: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/07467-1 - CeTICS - Centro de Toxinas, Imuno-Resposta e Sinalização Celular
• Beneficiário: Hugo Aguirre Armelin
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/13548-4 - Caracterização proteômica / glicoproteômica de venenos de serpentes do complexo Bothrops jararaca com ênfase no N-terminoma e N-glicoma de toxinas
• Beneficiário: Solange Maria de Toledo Serrano
• Linha de fomento: Auxílio à Pesquisa - Regular