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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ontogenetic Variation in Biological Activities of Venoms from Hybrids between Bothrops erythromelas and Bothrops neuwiedi Snakes

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Author(s):
Santoro, Marcelo Larami [1] ; do Carmo, Thais [1] ; Lopes Cunha, Bruna Heloisa [1] ; Alves, Andre Fonseca [1] ; Zelanis, Andre [2, 3] ; de Toledo Serrano, Solange Maria [2, 3] ; Grego, Kathleen Fernandes [4] ; Sant'Anna, Savio Stefanini [4] ; Barbaro, Katia Cristina [5] ; Fernandes, Wilson [4]
Total Authors: 10
Affiliation:
[1] Inst Butantan, Lab Fisiopatol, Sao Paulo, SP - Brazil
[2] Inst Butantan, Lab Especial Toxinol Aplicada, Sao Paulo, SP - Brazil
[3] Inst Butantan, Ctr Toxins Immune Response & Cell Signaling, Sao Paulo, SP - Brazil
[4] Inst Butantan, Lab Herpetol, Sao Paulo, SP - Brazil
[5] Inst Butantan, Lab Imunopatol, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PLoS One; v. 10, n. 12 DEC 29 2015.
Web of Science Citations: 5
Abstract

Lance-headed snakes are found in Central and South America, and they account for most snakebites in Brazil. The phylogeny of South American pitvipers has been reviewed, and the presence of natural and non-natural hybrids between different species of Bothrops snakes demonstrates that reproductive isolation of several species is still incomplete. The present study aimed to analyze the biological features, particularly the thrombin-like activity, of venoms from hybrids born in captivity, from the mating of a female Bothrops erythromelas and a male Bothrops neuwiedi, two species whose venoms are known to display ontogenetic variation. Proteolytic activity on azocoll and amidolytic activity on N-benzoyl-DL-arginine-p-nitroanilide hydrochloride (BAPNA) were lowest when hybrids were 3 months old, and increased over body growth, reaching values similar to those of the father when hybrids were 12 months old. The clotting activity on plasma diminished as hybrids grew; venoms from 3- and 6-months old hybrids showed low clotting activity on fibrinogen (i.e., thrombinlike activity), like the mother venom, and such activity was detected only when hybrids were older than 1 year of age. Altogether, these results point out that venom features in hybrid snakes are genetically controlled during the ontogenetic development. Despite the presence of the thrombin-like enzyme gene(s) in hybrid snakes, they are silenced during the first six months of life. (AU)

FAPESP's process: 08/02996-8 - Venoms from neonate and mature Bothrops jararaca snakes: comparison between enzymatic and biological activities, immunoneutralization, chemical composition and ability to induce hemostatic disorders
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants
FAPESP's process: 10/08162-1 - Contribution of local injury evoked by Bothrops jararaca snake venom to the genesis of systemic hemostatic disturbances
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants
FAPESP's process: 13/13548-4 - Proteomic/Glycoproteomic characterization of the venoms of the Bothrops jararaca complex with emphasis on the N-terminome and N-glycome of toxins
Grantee:Solange Maria de Toledo Serrano
Support type: Regular Research Grants
FAPESP's process: 09/15413-3 - Characterization and biological activities of venoms from adult Bothrops erythromelas and Bothrops neuwiedi and their hybrid offspring.
Grantee:Thaís Do Carmo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 13/25177-0 - Involvement of Von Willebrand factor in hemostatic disturbances induced by Bothrops jararaca snake venom: experimental study
Grantee:Marcelo Larami Santoro
Support type: Regular Research Grants
FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
Grantee:Hugo Aguirre Armelin
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC