Involvement of Von Willebrand factor in hemostatic disturbances induced by Bothrops jararaca snake venom: experimental study

• Grant number: 13/25177-0
• Support type: Regular Research Grants
• Duration: January 01, 2015 - December 31, 2017
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Marcelo Larami Santoro
• Grantee: Marcelo Larami Santoro
• Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Assoc. researchers: José Ricardo Jensen ; Katia Cristina Barbaro Nogueira ; Vania Gomes de Moura Mattaraia
• Associated grant(s): 16/50411-5 - Isolation and characterization of Brothrops jararaca snake venom proteins that induce thrombocytopenia , AP.R SPRINT

Abstract

Bothrops jararaca snake venom disrupts various biological systems in a complex and dynamic manner, causing thereby the rapid death of natural preys. Among these biological systems, the hemostatic system is a crucial target for snake venoms; particularly, B. jararaca venom has various pro- and anti-hemostatic activities. Given the wide range of hemostatic activities found in this venom, bleeding disorders occurring in patients are multifactorial. However, it is intriguing that some important features of hemorrhagic manifestations presented by patients bitten by B. jararaca, e.g. gingival bleeding, epistaxis, petechiae and suffusions, resemble those observed in patients with von Willebrand disease. Likewise, although the venom induces thrombocytopenia in envenomed patients, it is unknown whether von Willebrand factor (vWF) is involved in such process. Thus, this project aims: (1) to analyze the occurrence of quantitative and qualitative changes of vWF and its cleavage enzyme, ADAMTS13, during experimental envenomation by B. jararaca; (2) to investigate the role of vWF in the development of venom-induced thrombocytopenia. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS DE OLIVEIRA, PERCILLIA VICTORIA; GARCIA-ROSA, SHEILA; AZEVEDO SACHETTO, ANA TERESA; SOARES MORETTI, ANA IOCHABEL; DEBBAS, VICTOR; DE BESSA, TIPHANY CORALIE; SILVA, NATHALIA TENGUAN; PEREIRA, ALEXANDRE DA COSTA; MARTINS-DE-SOUZA, DANIEL; SANTORO, MARCELO LARAMI; MARTINS LAURINDO, FRANCISCO RAFAEL. Protein disulfide isomerase plasma levels in healthy humans reveal proteomic signatures involved in contrasting endothelial phenotypes. REDOX BIOLOGY, v. 22, APR 2019. Web of Science Citations: 1.

MALAQUE, C. M. S.; DUAYER, I. F.; SANTORO, M. L. Acute kidney injury induced by thrombotic microangiopathy in two cases of Bothrops envenomation. CLINICAL TOXICOLOGY, v. 57, n. 3, p. 213-216, MAR 4 2019. Web of Science Citations: 0.

ROSA, JAQUELINE GOMES; DE ALBUQUERQUE, CYNTHIA ZACCANINI; DE MOURA MATTARAIA, VANIA GOMES; SANTORO, MARCELO LARAMI. Comparative study of platelet aggregation and secretion induced by Bothrops jararaca snake venom and thrombin. Toxicon, v. 159, p. 50-60, MAR 1 2019. Web of Science Citations: 1.

THOMAZINI, CAMILA MARTOS; SILVA SOARES, ROSANGELA DE PAULA; FLORES DA ROCHA, TANIA RUBIA; AZEVEDO SACHETTO, ANA TERESA; SANTORO, MARCELO LARAMI. Optimization of von Willebrand factor multimer analysis in vertical mini-gel electrophoresis systems: A rapid procedure. THROMBOSIS RESEARCH, v. 175, p. 76-83, MAR 2019. Web of Science Citations: 0.

AZEVEDO SACHETTO, ANA TERESA; ROSA, JAQUELINE GOMES; SANTORO, MARCELO LARAMI. Rutin (quercetin-3-rutinoside) modulates the hemostatic disturbances and redox imbalance induced by Bothrops jararaca snake venom in mice. PLoS Neglected Tropical Diseases, v. 12, n. 10 OCT 2018. Web of Science Citations: 4.

AGUSTINA QUINTANA, MARIA; SCIANI, JULIANA MOZER; VATTI AUADA, ALINE VIVIAN; MERCEDES MARTINEZ, MARIA; NICOLAS SANCHEZ, MAGAS; SANTORO, MARCELO LARAMI; FAN, HUI WEN; ELISA PEICHOTO, MARIA. Stinging caterpillars from the genera Podalia, Leucanella and Lonomia in Misiones, Argentina: A preliminary comparative approach to understand their toxicity. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 202, p. 55-62, NOV 2017. Web of Science Citations: 7.

RODRIGUES, MARIANA VALOTTA; DE CASTRO, SIMONE OLIVEIRA; DE ALBUQUERQUE, CYNTHIA ZACCANINI; DE MOURA MATTARAIA, VANIA GOMES; SANTORO, MARCELO LARAMI. The gingival vein as a minimally traumatic site for multiple blood sampling in guinea pigs and hamsters. PLoS One, v. 12, n. 5 MAY 22 2017. Web of Science Citations: 1.

ELISA PEICHOTO, MARIA; SANTORO, MARCELO LARAMI. Patagonfibrase modifies protein expression of tissue factor and protein disulfide isomerase in rat skin. Toxicon, v. 119, p. 330-335, SEP 1 2016. Web of Science Citations: 4.

SANTORO, MARCELO LARAMI; DO CARMO, THAIS; LOPES CUNHA, BRUNA HELOISA; ALVES, ANDRE FONSECA; ZELANIS, ANDRE; DE TOLEDO SERRANO, SOLANGE MARIA; GREGO, KATHLEEN FERNANDES; SANT'ANNA, SAVIO STEFANINI; BARBARO, KATIA CRISTINA; FERNANDES, WILSON. Ontogenetic Variation in Biological Activities of Venoms from Hybrids between Bothrops erythromelas and Bothrops neuwiedi Snakes. PLoS One, v. 10, n. 12 DEC 29 2015. Web of Science Citations: 5.