Antibodies Against Glycolipids Enhance Antifungal Activity of Macrophages and Reduce Fungal Burden After Infection with Paracoccidioides brasiliensis

Autor(es)

Bueno, Renata A. ^{[1, 2]} ; Thomaz, Luciana ^[1] ; Munoz, Julian E. ^[1] ; da Silva, Cassia J. ^[1] ; Nosanchuk, Joshua D. ^{[3, 4]} ; Pinto, Marcia R. ^[5] ; Travassos, Luiz R. ^[6] ; Taborda, Carlos P. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Lab Med Mycol IMTSP LIM53, Sao Paulo - Brazil

^[3] Albert Einstein Coll Med, Dept Med, New York, NY - USA

^[4] Albert Einstein Coll Med, Dept Microbiol & Immunol, New York, NY - USA

^[5] Univ Fed Fluminense, Niteroi, RJ - Brazil

^[6] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN MICROBIOLOGY; v. 7, FEB 3 2016.
• Citações Web of Science: 2

Resumo

Paracoccidioidomycosis is a fungal disease endemic in Latin America. Polyclonal antibodies to acidic glycosphingolipids (GSLs) from Paracoccidioides brasiliensis opsonized yeast forms in vitro increasing phagocytosis and reduced the fungal burden of infected animals. Antibodies to GSL were active in both prophylactic and therapeutic protocols using a murine intratracheal infection model. Pathological examination of the lungs of animals treated with antibodies to GSL showed well-organized granulomas and minimally damaged parenchyma compared to the untreated control. Murine peritoneal macrophages activated by IFN-gamma and incubated with antibodies against acidic GSLs more effectively phagocytosed and killed P brasiliensis yeast cells as well as produced more nitric oxide compared to controls. The present work discloses a novel target of protective antibodies against P brasiliensis adding to other well-studied mediators of the immune response to this fungus. (AU)

• Processo FAPESP: 11/17267-4 - Avaliação de uma vacina baseada no peptídeo-P10 e seu efeito terapêutico quando administrado em combinação com antifúngicos no modelo da fibrose experimental induzido com conídios de Paracoccidioides Brasiliensis
• Beneficiário: Carlos Pelleschi Taborda
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/18655-3 - Paracoccidioides lutzii: desenvolvimento de vacina terapêutica a partir de antígenos específicos para serem utilizados em vacina combinada com o peptídeo P10 de P. brasiliensis
• Beneficiário: Carlos Pelleschi Taborda
• Modalidade de apoio: Auxílio à Pesquisa - Regular