Myeloperoxidase in human peripheral blood lymphocytes: Production and subcellular localization

Autor(es)

Okada, Sabrina Sayori ^[1] ; de Oliveira, Edson Mendes ^[1] ; de Araujo, Tomaz Henrique ^[2] ; Rodrigues, Maria Rita ^[3] ; Albuquerque, Renata Chaves ^[1] ; Mortara, Renato Arruda ^[4] ; Taniwaki, Noemi Nosomi ^[5] ; Nakaya, Helder Imoto ^{[1, 6]} ; Campa, Ana ^[1] ; Ramos Moreno, Ana Carolina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508 Sao Paulo, SP - Brazil

^[2] Univ Fed Alfenas, Dept Ciencias Biol, BR-37130 Alfenas, MG - Brazil

^[3] Univ Fed Alfenas, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-37130 Alfenas, MG - Brazil

^[4] Univ Fed Sao Paulo, Dept Microbiol Imunobiol & Parasitol, BR-04023 Sao Paulo, SP - Brazil

^[5] Adolfo Lutz Inst, Nucleo Microscopia Eletron, BR-01246 Sao Paulo, SP - Brazil

^[6] Emory Univ, Yerkes Natl Primate Res Ctr, Emory Vaccine Ctr, Atlanta, GA 30329 - USA

• Tipo de documento: Artigo Científico
• Fonte: Cellular Immunology; v. 300, p. 18-25, FEB 2016.
• Citações Web of Science: 5

Resumo

Myeloperoxidase (MPO) is an important enzyme in the front-line protection against microorganisms. In peripheral blood, it is accepted that MPO is only produced by myeloid-lineage cells. Thus, MPO presence is unexpected in lymphocytes. We showed recently that BI-lymphocytes from mice have MPO. Here, we showed that subsets of human peripheral B, CD4(+) and CD8(+) T lymphocytes express MPO. The content of MPO in lymphocytes was very low compared to neutrophils/monocytes with a preferential distribution in the nucleus and perinuclear region. Also, we performed a MPO mRNA expression analysis from human blood cells derived from microarray raw data publicly available, showing that MPO is modulated in infectious disease. MPO was increased in CD4(+) T lymphocytes from HIV chronic infection and in CD8(+) T lymphocytes from HCV-positive patients. Our study points out MPO as a multifunctional protein due to its subcellular localization and expression modulation in lymphocytes indicating alternative unknown functions for MPO in lymphocytes. (C) 2015 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 06/50641-9 - Efeito da interação entre macrófagos e neutrófilos na expressão, atividade e localização do sistema NADPH oxidase e das enzimas mieloperoxidase e indolamina 2,3 diaxigenase
• Beneficiário: Sabrina Sayori Okada Matino
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 09/14632-3 - Via das quinureninas versus via serotonérgica: participação na comunicação entre células do sistema imune e no imune escape de tumores
• Beneficiário: Ana Campa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/54474-5 - Influencia do metabolismo de triptofano no imune escape: efeitos na comunicacao entre celulas tumorais, dendriticas, macrofagos e linfocitos.
• Beneficiário: Ana Carolina Ramos Moreno
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/18498-7 - Novas perspectivas para o papel de amilóide sérica A (SAA) na obesidade e resistência à insulina
• Beneficiário: Edson Mendes de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado