Safety and efficacy evaluation of gelatin-based nanoparticles associated with UV filters

Autor(es)

de Oliveira, Camila Areias ^[1] ; Dario, Michelli Ferrera ^[1] ; Sarruf, Fernanda Daud ^[2] ; Afonso Mariz, Ines Fatima ^{[3, 4]} ; Robles Velasco, Maria Valeria ^[1] ; Rosado, Catarina ^[5] ; Baby, Andre Rolim ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharm, Lab Cosmetol, 580 Prof Lineu Prestes, Av Bl 15, BR-05508900 Sao Paulo, SP - Brazil

^[2] Clin Res Co, Pesquisa Integrada, IPclin, Sao Paulo, SP - Brazil

^[3] Univ Lisbon, Inst Super Tecn, CQFM, Av Rovisco Pais 1, P-1049001 Lisbon - Portugal

^[4] Univ Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol IN, Av Rovisco Pais 1, P-1049001 Lisbon - Portugal

^[5] Univ Lusofona, Res Ctr Biosci & Hlth Technol, CBIOS, Lisbon - Portugal

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 140, p. 531-537, APR 1 2016.
• Citações Web of Science: 12

Resumo

The safety and efficacy assessment of nanomaterials is a major concern of industry and academia. These materials, due to their nanoscale size, can have chemical, physical, and biological properties that differ from those of their larger counterparts. The encapsulation of natural ingredients can provide marked improvements in sun protection efficacy. This strategy promotes solubility enhancement of flavonoids and yields an improved active ingredient with innovative physical, physicochemical and functional characteristics. Rutin, a flavonoid, has chemical and functional stability in topical vehicles exerting a synergistic effect in association with ultraviolet (UV) filters. However, the solubility of rutin is a limiting factor. Additionally, this bioactive compound does not have tendency to permeate across the stratum corneum. As an alternative to common synthetic based sunscreens, rutin-entrapped gelatin nanoparticles were designed. The present study investigated the pre-clinical safety of gelatin nanoparticles (GNPs) using an in vitro method and also assessed the clinical safety and efficacy of the association of GNPs with three commonly used chemical UV filters (ethylhexyl dimethyl PABA, ethylhexyl methoxycinnamate and methoxydibenzoylmethane). The non-irritant and adequate safety profile under sun-exposed skin conditions of the nanomaterials and the emulsions qualified the products for clinical efficacy assays. The in vivo results indicated that the GNPs increased the antioxidant protection of the emulsions developed. However, the presence of rutin in the nanosized material did not enhance performance on the SPF test. In conclusion, these findings characterized the nanomaterials as an innovative platform for multifunctional bioactive sunscreens. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/05906-0 - Estabelecimento das propriedades de segurança e eficácia clínica cosmética de sistemas nanoestruturados de base tecnológica proteica associados à rutina
• Beneficiário: Camila Areias de Oliveira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 12/19972-0 - Desenvolvimento tecnológico, avaliação da segurança e eficácia clínica de sistemas micro/nanoparticulados de gelatina contendo rutina
• Beneficiário: Camila Areias de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 12/04435-9 - Sistemas particulados de gelatina contendo flavonóide: desenvolvimento, caracterização e aplicação em formulações antissolares
• Beneficiário: André Rolim Baby
• Modalidade de apoio: Auxílio à Pesquisa - Regular