Maternal periodontitis decreases plasma membrane GLUT4 content in skeletal muscle of adult offspring

Autor(es):Mostrar menos -

de Lima Coutinho Mattera, Maria Sara ^[1] ; Chiba, Fernando Yamamoto ^[2] ; de Oliveira da Mota, Max Sander ^[1] ; Pereira, Renato Felipe ^[1] ; Ervolino, Edilson ^[1] ; Chaves Neto, Antonio Hernandes ^[1] ; Pescinini Salzedas, Leda Maria ^[3] ; Scaramele, Natalia Francisco ^[1] ; Silva, Cristina Antoniali ^[1] ; Okamoto, Maristela Mitiko ^[4] ; Machado, Ubiratan Fabres ^[4] ; Sumida, Doris Hissako ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Dent Sch Aracatuba, Program Posgrad Multictr Ciencias Fisiol SBFis, Dept Basic Sci, UNESP, BR-16015050 Aracatuba, SP - Brazil

^[2] Univ Estadual Paulista, Dent Sch Aracatuba, Prevent & Social Dent Postgrad Program, UNESP, BR-16015050 Aracatuba, SP - Brazil

^[3] Univ Estadual Paulista, Dent Sch Aracatuba, Dept Pathol & Clin Propedeut, UNESP, BR-16015050 Aracatuba, SP - Brazil

^[4] Univ Sao Paulo, Sao Paulo Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 148, p. 194-200, MAR 1 2016.
• Citações Web of Science: 3

Resumo

Aims: The fetal programming hypothesis suggests that intrauterine stimuli can induce metabolic changes in offspring, increasing the disease risk in adulthood. Periodontal disease may enhance serumcytokine levels. Cytokines such as tumor necrosis factor-alpha (TNF-alpha) have been associatedwith reduced glucose transporter type 4 (GLUT4) expression, decreased protein kinase B (Akt) phosphorylation, and insulin resistance. This study aimed to evaluate GLUT4 content, and Akt serine phosphorylation status in the gastrocnemius skeletal muscle (GSM), glycemia, insulinemia and change in body weight in offspring of rats with periodontal disease. Main methods: Female Wistar rats were distributed into a control group (CN) and an experimental periodontal disease group (PD), inwhich a ligature was placed around themandibular firstmolars. Seven days after ligature placement, both groups were mated with normal male rats. The ligatures remained throughout pregnancy until weaning, after which the male offspring were distributed into groups: CN-o, control rat offspring; and PD-o, periodontal disease rat offspring. The body weight from 0 to 75 days of age wasmeasured. At 75 days, the glycemia, insulinemia, TNF-alpha levels, Akt serine phosphorylation, and GLUT4 content in the GSMweremeasured in the offspring. Key findings: The PD-o group showed a low birth weight (LBW), unchanged glycemia, increased insulinemia, insulin resistance, increased TNF-alpha levels, decreased Akt serine phosphorylation status, and reduced GLUT4 content in the plasma membrane and translocation index after insulin stimulation. Significance: Maternal periodontal disease causes LBW, insulin resistance, and alterations in the final stage of insulin signaling in the GSM of adult offspring. (C) 2016 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/08783-4 - Avaliação do conteúdo de glut4 e seu índice de translocação para membrana plasmática em tecido muscular de ratos adultos, proles de ratas com doença periodontal
• Beneficiário: Maria Sara de Lima Coutinho Mattera
• Modalidade de apoio: Bolsas no Brasil - Mestrado