Light-emitting diode therapy increases collagen deposition during the repair process of skeletal muscle

Autor(es)

Viana de Melo, Claudia Aparecida ^[1] ; Alves, Agnelo Neves ^[1] ; Lins Terena, Stella Maris ^[2] ; Santos Fernandes, Kristianne Porta ^{[2, 1]} ; Nunes, Fabio Daumas ^[3] ; Teixeira da Silva, Daniela de Fatima ^[2] ; Bussadori, Sandra Kalil ^{[2, 1]} ; Deana, Alessandro Melo ^[2] ; Mesquita-Ferrari, Raquel Agnelli ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 31, n. 3, p. 531-538, APR 2016.
• Citações Web of Science: 2

Resumo

This study analyzed the effects of light-emitting diode (LED) therapy on the morphology of muscle tissue as well as collagen remodeling and matrix metalloproteinase 2 (MMP-2) activity in the skeletal muscle of rats following acute injury. Wistar rats were divided into four groups: (1) control, (2) sham, (3) untreated cryoinjury, and (4) cryoinjury treated with LED. Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly onto the belly of the tibialis anterior muscle. For treatment, the LED equipment (wavelength 850 nm, output power 30 mW, and total energy 3.2 J) was used daily. The study periods were 1, 3, and 7 days after cryoinjury. Morphological aspects were evaluated through hematoxylin-eosin staining. The amount of collagen fibers was evaluated using Picro Sirius Red staining under polarized light. The gelatinase activity of MMP-2 was evaluated using zymography. The results showed significant reductions in inflammatory infiltrate after 3 days and an increased number of immature muscle fibers after 7 days. Furthermore, treatment induced a reduction in the gelatinolytic activity of MMP-2 after 1, 3, and 7 days in comparison to the untreated injury groups and increased the collagen deposition after 3 and 7 days in the treated groups. LED therapy at 850 nm induced a significant reduction in inflammation, decreased MMP-2 activity, and increased the amount of immature muscle and collagen fibers during the muscle repair process following acute injury. (AU)

• Processo FAPESP: 14/12381-1 - Análise comparativa do efeito da laserterapia prévia associada ou não ao tratamento pós sobre a modulação de citocinas inflamatórias, do estresse oxidativo e a determinação do tipo de fibra muscular após lesão aguda
• Beneficiário: Raquel Agnelli Mesquita Ferrari
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/21540-3 - Efeito comparativo da terapia laser de baixa potência e do ultrassom da baixa intensidade sobre o fenótipo de fibras musculares, estresse oxidativo e proteínas envolvidas na regulação do reparo muscular
• Beneficiário: Agnelo Neves Alves
• Modalidade de apoio: Bolsas no Brasil - Doutorado