| Full text | |
| Author(s): |
Viana de Melo, Claudia Aparecida
[1]
;
Alves, Agnelo Neves
[1]
;
Lins Terena, Stella Maris
[2]
;
Santos Fernandes, Kristianne Porta
[2, 1]
;
Nunes, Fabio Daumas
[3]
;
Teixeira da Silva, Daniela de Fatima
[2]
;
Bussadori, Sandra Kalil
[2, 1]
;
Deana, Alessandro Melo
[2]
;
Mesquita-Ferrari, Raquel Agnelli
[2, 1]
Total Authors: 9
|
| Affiliation: | [1] Univ Nove Julho UNINOVE, Postgrad Program Rehabil Sci, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil
[2] Univ Nove Julho UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Lasers in Medical Science; v. 31, n. 3, p. 531-538, APR 2016. |
| Web of Science Citations: | 2 |
| Abstract | |
This study analyzed the effects of light-emitting diode (LED) therapy on the morphology of muscle tissue as well as collagen remodeling and matrix metalloproteinase 2 (MMP-2) activity in the skeletal muscle of rats following acute injury. Wistar rats were divided into four groups: (1) control, (2) sham, (3) untreated cryoinjury, and (4) cryoinjury treated with LED. Cryoinjury was induced by two applications of a metal probe cooled in liquid nitrogen directly onto the belly of the tibialis anterior muscle. For treatment, the LED equipment (wavelength 850 nm, output power 30 mW, and total energy 3.2 J) was used daily. The study periods were 1, 3, and 7 days after cryoinjury. Morphological aspects were evaluated through hematoxylin-eosin staining. The amount of collagen fibers was evaluated using Picro Sirius Red staining under polarized light. The gelatinase activity of MMP-2 was evaluated using zymography. The results showed significant reductions in inflammatory infiltrate after 3 days and an increased number of immature muscle fibers after 7 days. Furthermore, treatment induced a reduction in the gelatinolytic activity of MMP-2 after 1, 3, and 7 days in comparison to the untreated injury groups and increased the collagen deposition after 3 and 7 days in the treated groups. LED therapy at 850 nm induced a significant reduction in inflammation, decreased MMP-2 activity, and increased the amount of immature muscle and collagen fibers during the muscle repair process following acute injury. (AU) | |
| FAPESP's process: | 14/12381-1 - Comparative analysis of the effect of previous laser therapy associated or not to post treatment on the modulation of inflammatory cytokines, oxidative stress and the determination of muscle fiber type after acute injury |
| Grantee: | Raquel Agnelli Mesquita Ferrari |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/21540-3 - Comparative effect of low-level laser therapy and low intensity ultrasound on myofiber phenotypes, oxidative stress and proteins during muscle repair |
| Grantee: | Agnelo Neves Alves |
| Support Opportunities: | Scholarships in Brazil - Doctorate |